文蛤粗多肽对免疫抑制小鼠TNF-α,IL-2及IFN-γ的影响

Effect of Meretrix Meretrix Linnaeus crude polypeptide on TNF-α,IL-2 and IFN-γof immunosuppressive mice

目的:研究文蛤粗多肽的免疫药理活性,为后期产品开发提供研究基础。方法:取雄性KM小鼠50只,随机分为5组:空白组、模型组、左旋咪唑组(1.0 mg/kg)、文蛤粗多肽高剂量组(200 mg/kg)、文蛤粗多肽低剂量组(100 mg/kg),每组10只。灌胃给药,每日1次,连续给药30 d,灌胃体积为0.2 ml/10 g BW。同时,除空白组外,其余各组小鼠每隔2日灌服地塞米松(DXM,1.5 mg/kg)1次进行免疫抑制造模,共15次。末次灌胃6 h后,称重,摘眼球取血,脱颈椎处死小鼠,并取脏器,测定免疫器官指数与血清中TNF-α,IL-2与IFN-γ的变化。结果:文蛤粗多肽对免疫抑制小鼠胸腺指数、脾脏指数与体重具有明显增加作用(P<0.05);并可明显升高免疫抑制小鼠血清中TNF-α、IL-2与IFN-γ的水平(P<0.05)。结论:文蛤粗多肽对地塞米松造成的免疫抑制小鼠具有免疫增强作用。

Objective：To study the immune pharmacological bioactivities of Meretrix Meretrix Linnaeus crude polypeptide,providing research foundation for the product development in the future. Methods： Fifty Kunming male mice were divided into control group, model group, positive group （Levamisole 1.0 mg/kg）, high-dose group （Mere- trix Meretrix Linnaeus 200 mg/kg） and low-dose group （Meretrix Meretrix Linnaeus 100 mg/kg） with 10 mice in each group. These groups were given relevant drugs with 0.2 ml/10 g BW once a day for 30 days. Meanwhile,except for control group,mice in each group were fed with dexamethasone 1.5 mg/kg every second day to establish im- munosuppressive model for 15 times. The mice were weighed with eyeball removing for blood after 6 h from the last garage. Then, they were sacrificed with cervical spine taking off for collecting organs. The variation of TNF-α, IL-2 and IFN-γ in serum and index in immune organ were determined. Result： Meretrix Meretrix Linnaeus crude polypeptide could significantly enhance the thymus index, spleen index and weight of DXM immunosuppressive mice （P〈0.05）, and could increase the levels of TNF-α, IL-2 and IFN-γ in serum （P〈0.05）. Conclusion： Meretrix Meretrix Linnaeus crude polypeptide has the function of immune enhancement for DXM immunosuppressive mice.