青霉素发酵液中生物表面活性剂的提取

Abstraction of Bio-surfactants from Penicillin Broth

采用超滤法分离青霉素发酵液中的表面活性物质,并将表面活性物质富集浓缩,再结合冷冻干燥等手段回收,得到可利用的生物表面活性剂,同时解决了青霉素发酵液乳化的问题。截留分子量为10kDa的超滤膜能有效分离料液中的表面活性物质,每升发酵液中含表面活性剂2.659g。

In this paper we use the method of ultrafiltration to get the surfactants from penicillin broth.By this means,we solve the long-lasting problem of emulsification in the solvent extraction process.At the same time these surface-active substances are concentrated by UF,and then collected by freeze-drying. It is shown that the UF membrane with an MWCO of 10 kD could remove the surfactants from the broth efficiently,and thereby eliminate the emulsification in the solvent extraction process.There is 2.659 g such biosurfactant per liter antibiotic broth.Besides,we also study how the temperature and pressure could affect the UF membrane＇s performance,discuss the mechanism of the membrane-contamination,and clean the UF membrane with different methods and under different temperature to decide the best cleanout method and manipulative meters：At 35℃,flushing the UF membrane with pure water under manipulative pressure first,then inverting the membrane and flushing with pure water again,then cleaning the membrane with 0.4% NaOH solution,finally flushing with pure water till litmus less.