不同色价山葡萄总酚及相关酶活性的比较分析

Comparison of the Total Phenol and Related Enzymes in Amur Grape of Different Color Unite

以14份山葡萄品系为试材,根据果汁颜色的差异,应用方差分析法和相关性分析法,对14份果汁颜色深浅不同的山葡萄资源的色价值、总酚含量和苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）活性进行了比较分析。结果表明：14份山葡萄资源果实色价值变化幅度为5.889~62.861g-1;其果实色价值、总酚、PAL及PPO活性存在明显差异;色价值与总酚及PAL、PPO活性呈显著正相关;总酚含量又与PAL和PPO活性呈正相关。由此可见,果汁颜色越深的山葡萄资源,果实总酚含量越高,说明其具有的抗氧化能力较强;但由于其较高的PPO活性,在加工过程中应控制褐变的发生。

Taking 14 selected amur grape resources as materials, comparison of color unite, total phenol and related enzymes were carried out by variance analysis and correlation analysis according to the differences in juice color. The results showed that range of the color unite was 5. 889-62. 861 g-1 ;the color unite, total phenol, PAL and PPO activities of 14 amur grape resources were significant difference content of color unite showed a positive correlation with total phenol and the activity of PAL,PPO. Meanwhile,the content of total phenol was positive correlation with PAL and PPO activity. Thus it could be seen that the deeper juice color,the higher total phenol in fruit which would be more stronger antioxidant ability. However,because of the higher PPO activity in deeper juice color of amur grape,the browning should be controlled in the process.