西芹水提液对蚕豆根尖微核率的影响

Effect of Water Extract of Apium graveolens on Micronucleus Rate of Vicia Faba Root Tip Cells

以蚕豆为试材,采用根尖细胞微核试验方法,研究了不同浓度的西芹水提液与未添加和添加环磷酰胺溶液对蚕豆根尖微核率的影响。结果表明：未添加环磷酰胺溶液时,不同浓度的西芹水提液对蚕豆根尖细胞微核率的产生没有诱导作用,平均微核率无明显变化,微核指数保持在1.3左右;添加环磷酰胺溶液时,西芹水提液对环磷酰胺诱导的蚕豆根尖微核率的抑制作用较明显,抑制率为52%~72%,且与其浓度存在剂量-反应关系,相关系数r=0.9048,其抑制作用随着西芹水提液浓度的增大而增强,表明西芹水提液具有一定的抗突变作用。

Taking Vicia faba as experimental materials, using the method of micronucleus test in root tip cells, effect of water extract of Apiurn graveolens on micronucleus rate of Vicia fdba root tips treated with cyclophosphamide and without cyelophosphamide were studied. The results showed that without cyclophosphamide,different concentrations of water extract of Apium graveolens did not induce the formation of micronucleus of Vicia faba root tips, average micronucleus rate had no significant change,the micronucleus index kept at around 1.3; with cyclophosphamide, water extract of Apium graveolens could effectively inhibit micronucleus rate of Vicia faba root tip cells induced by cyclophosphamide and the rate of inhibitions was 52%- 72%, which was in dose-effect relationship with the concentration of water extract of Apium graveolens, r = 0. 9048. The inhibitory action of the water extract of Apium graveolens could be strengthened along with the increasing concentrations of itself, which indicated the water extract of Apium graveolens had some antimutation function.