摘要
以花椒提取物为试材,采用生长速率法测定了不同浓度的花椒提取物对桃褐腐病菌的抑制效果;并对处理后桃果实的苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)活性进行检测。结果表明:不同浓度的花椒提取物对褐腐病菌菌丝的生长表现出较强的抑制效果,浓度为5.00mg/mL时抑制效果最好,抑制生长率达到96.71%,其EC50为0.504mg/mL;桃果实经提取物处理后,能显著诱导果实中抗病相关酶PAL、POD、PPO活性升高,并在整个试验过程中保持较高的水平。花椒提取物对桃褐腐病菌具有一定的抑制作用和诱导桃果实产生抗性的作用。
Taking extract of Zanthoxylum bungeanum as material,the mycelium growth rate method was used to test the inhibitory effect of extracts with different concentrations on Monilinia fructicola the activities of phenylalanine ammoniumlyase（PAL） ,peroxidease（POD）and polyphenol oxidase（PPO）induced by inoculation with extract were studied in peach. The results showed that the extract of Zanthoxylurn bungeanum displayed strong antifungal activity to M. fructicola. When the concentration of extracts was 5. 00 mg/mL, the inhibitory effect was best the inhibitory rate was 96. 71%. The EC^0 value was 0. 504 mg/mL. The activities of PAL,POD and PPO in peach increased significantly when inoculated with extract and M. fructicola, and maintained at a high level throughout the experiment. The extract of Zanthoxylurn bungeanurn had antifungal activity and exerted a potent impact on inducing resistance of peach to M. fructicola.
出处
《北方园艺》
CAS
北大核心
2014年第20期129-132,共4页
Northern Horticulture
基金
黔西南州科技局计划资助项目(2010-14)
