食品中单核细胞增生性李斯特氏菌耐药性与分子分型研究

Antibiotic resistance and subtyping on Listeria Monocytogenes from foods

目的了解单核细胞增生性李斯特氏菌(L.monocytogenes LM)的耐药性质以及分子分型的具体特点,探讨耐药表型与PFGE基因组型之间所具备哪些关联。方法采取自2011—2013年期间我市各区的熟食、牲畜肉类、以及海鲜水产品等食品中采取80株LM,采用微量肉汤稀释法观察抗生素的敏感性。结果发现16.25%(13/80)的菌株存在耐药。结论在所选取的LM存在一定的耐药性,LM的PFGE分型与其药敏分型无对应关系。

Objective To investigate the Listeria monocytogenes List Prand（L.monocytogenes LM） the specific characteristics of resistant properties and molecular typing, discuss between resistant phenotype and PFGEgenotypes have what relation. Methods From 2011-2013 year my city district of cooked food, livestock meat, and seafood aquatic products and other food to 80 strains of LM, the sensitivity of antibiotics by the broth microdilutionmethod. Results 16.25%（13/80） strains have resistance. Conclusion There arecertain resistance in the selected LM, LM, PFGE typing and drug susceptibility typing without corresponding relationship.