艾滋病患者肠道微生态与CD4^+T淋巴细胞计数的关系

A preliminary study on intestinal microflora of AIDS patients and its correlation with CD4^+ T lymphocytes.

目的分析艾滋病患者肠道微生态的变化,并研究其与CD4+T淋巴细胞计数的关系。方法 59例艾滋病患者作为观察组,30例健康成人作为对照组,革兰染色涂片观察进行肠道菌群分度,选择肠道菌群中具有代表性的5种菌(双歧杆菌、肠球菌、肠杆菌、乳酸杆菌、真菌)进行细菌培养及计数,并计算反映肠道定植抗力的指标B/E值。结果两组肠道菌群分度差异有统计学意义(P<0.001),对照组的肠道菌群分度以正常的居多,观察组的以Ⅰ度的居多;5种代表菌,与对照组比较,观察组肠球菌及真菌数量增多(P<0.05);CD4+T淋巴细胞计数与肠球菌及肠道菌群分度呈负相关。结论艾滋病患者与正常人肠道微生态有差异,提示其肠道紊乱,且与CD4+T淋巴细胞计数存在相关性。

Objective To investigate the intestinal microecology in AIDS patients and its correlation with CD4^＋T lymphocytes. Methods A total of 89 cases were recruited into the cohort and divided into two groups： experimental group（AIDS patients,n =59） and control group（health adults,n =30）. Feces samples were collected. The intestinal florae were observed by gram stain. All feces samples were cultures under both aerobic and anaerobic conditions for four species of bacteria（Bifidobacterium,Enterococcus,Enterobacteriaceae and Lactobacillus） and the yeasts,the ratio of B /E was calculated as well. Results There was statistically significant difference between the two groups in intestinal flora index（P〈0.001）. The majority of the control group was normal,while the majority in the experimental group was found to be Degree I. Compared with control group,the counts of Enterococcus and yeasts in AIDS patients were significantly increased（P〈0. 05）. The CD4^＋T lymphocyte count was significantly negatively correlated with the Enterococcus count and the intestinal flora index. Conclusion The intestinal microflora of AIDS patients is obviously different with normal persons,indicating the disorder of intestinal microflora in AIDS patients. And this is correlated with the CD4^＋T lymphocyte count.