高效液相色谱法测定日化用品中甲醛的含量

RP-HPLC method for determination of formaldehyde in detergents

建立了柱前衍生、高效液相色谱法测定表面活性剂及其制品中甲醛含量的方法。乙酰丙酮作为衍生剂，铵存在的条件下与甲醛发生反应，衍生物直接进行液相分析，乙腈/水（体积比55：45）为流动相，流速1 mL/min。Agi-lent ZORBAX SB-C8（4.6 mm ×250 mm，5μm）为分离柱，在410 nm波长下进行检测。方法检出限0.002 mg/L，相对标准偏差0.73%~2.86%，回收率为97.9%~103.6%。该方法利用柱前衍生，生成稳定的黄色络合物，不需要进行萃取，操作简便、快速、准确性好。

A method of liquid chromatograPhy-diode array detector-Precolumn derivatization was set uP which can be used in determination of formaldehyde. Acetylacetone is the derivative agent,with ammoni-um,it can take reaction with formaldehyde. The derivatives detected straightly by HPLC. Mobile Phase：acetonitrile and water（V/V=55：45）,the flow rate is 1 mL/min. Agilent ZORBAX SB-C8（4. 6 mm×250 mm,5 μm）as the chromatograPhic column. The results show that detection wavelength at 410 nm, the limit of detection was 0. 002 mg/L,the relative standard deviation（ RSD）ranges from 0. 73% ~2. 86%,the recovery of the samPles were 97 . 9% ~ 103 . 6%. This method take use of Precolumn derivat-ization,generated steady yellow comPlex comPound without extraction. It is simPle,quick,and with good reProducibility.