益肾化浊解毒法对D-半乳糖致痴呆小鼠学习记忆及氧化应激的影响

Influence of Yishen Huazhuo detoxification method to learning and memory and oxidative stress in dementia mice due to D-galactose

目的:探讨益肾化浊解毒法对D-半乳糖致痴呆小鼠学习记忆障碍保护作用及对脑组织氧化应激的影响。方法:BALB/c小鼠颈背皮下注射D-半乳糖100 mg/kg BW·d×50 d,按随机数字表法分为模型组、阳性药(安理申)组、复方苁蓉益智胶囊高、中、低剂量组。以同月龄正常BALB/c小鼠为正常对照组。避暗实验测定小鼠学习记忆能力,比较各组小鼠脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性和丙二醛(MDA)含量及8-羟基-2’-脱氧鸟苷(8-OHdG)表达量。结果:复方苁蓉益智胶囊可显著增加D-半乳糖致痴呆小鼠进入暗室的潜伏期和减少错误次数,提高模型小鼠脑组织SOD、GSH-PX活性,降低脂质过氧化产物MDA含量和8-OhdG表达量。结论:复方苁蓉益智胶囊可改善D-半乳糖致痴呆小鼠的学习记忆能力,抑制脑组织内氧化应激,保护线粒体。

Objective： To investigate the effects of replenishing kidney, resolving turbidity and detoxifying on cognition and oxidative stress in D-galactose-induced dementia model of mice. Methods ： BALB/c mice were injected hypodermically with D-galactose in dosage of 100 mg/kg BW. d × 50 d, and were randomly divided into 5 groups, model, positive drug （Aricept） , high, medium and low dosage of Fufang Chongrong （FFCR）. BALB/c mice with same age were served as normal control. The ability of learning and memory of mice were examined by step through test. The activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-PX） were assayed. The level of malondialdehyde （MDA） and 8- hydroxy-2＇-deoxyguanosine （8-OHdG） in mice brain was evaluated. Results： FFCR improved cognition of D-galactose- induced demented mice by promoting activity of SOD and GSH-PX, and thus decreasing the level of MDA and 8-OhdG. Conclusion： FFCR could improve cognition and ameliorate oxidative stress in D-galactose-induced demented mice via mitochondrial protection.