HPLC法测定荔之咽滴丸中高车前苷含量

Determination of homoplantaginin content in the Lizhiyan dropping pills by HPLC

目的：建立荔之咽滴丸中高车前苷含量的高效液相色谱（HPLC）测定方法，为荔之咽滴丸的质量控制提供依据。方法色谱柱：Hedera ODS-2（250.0 mm×4.6 mm，5μm）；流动相：甲醇∶0.5%冰醋酸水溶液（50∶50）；柱温：30℃；流速：1.0 mL/min；检测波长：335 nm；进样量：10μL。结果高车前苷在0.20～2.52μg时呈良好的线性关系，相关系数（r）＝0.9998；平均回收率为99.80%，其相对标准偏差（RSD）为2.35%。结论 HPLC用于测定荔之咽滴丸中高车前苷含量，方法简便、准确、专属性强、重复性好，能起到控制荔之咽滴丸中高车前苷含量的作用。

Objective To establish determination method of homoplantaginin content in the Lizhiyan dropping pills by high performance liquid chromatography（HPLC）,in ordetr to provide a basis for the quality control of Lizhiyan dropping pills. Methods The chromatographic column：Hedera ODS-2（250.0 mm ×4.6 mm,5μm）;mobile phase：methanol∶0.5%glacial acetic acid（50∶50）;column temperature：30℃;flow rate：1.0 mL/min;detection wavelength：335 nm;injection volume：10μL. Results Homoplantaginin showed a good linear relationship when the injection volume was within 0.20-2.52 μg with the correlation coeffi-cient of 0.999 8;the average recovery rate was 99.8%,and the relative standard deviation （RSD） was 2.35%. Conclusion This method is simple,accurate,specific and repeatable,and it has contribution to control the content of homoplantaginin in the Lizhiyan dropping pills.