念珠菌性外阴阴道炎快速鉴定法的实用价值分析

Rapid Identification of Pathogenic Fungi in Patients with Candida Vulvovaginitis

目的：探讨念珠菌性外阴阴道炎患者的阴道分泌物采用直接提取DNA并进行PCR快速鉴定致病菌种的实用价值。方法收集我院2013年3月-2013年10月皮肤性病科门诊收治的念珠菌性外阴阴道炎患者阴道分泌物标本共50例，初步镜检鉴定为念珠菌，然后进行分泌物直接提取DNA，扩增其ITS1-2区，测序后确定菌种，并与其培养菌落鉴定结果进行比较。结果对50例念珠菌性阴道炎患者的阴道分泌物进行测序，直接分泌物提取法测序成功率为94%（47/50），SDA培养后测序成功率为64%（32/50），差异有统计学意义。结论采用阴道分泌物提取DNA进行PCR扩增后基因测序方法，可快速鉴定出常见的致病真菌，与传统的培养法相比，快速准确，具有一定的实用性。

Objective To identify pathogenic fungi rapidly through vaginal secretion samples from patients with Candida vulvovagi-nitis by direct DNA extraction and PCR. . Methods 50 vaginal secretion samples were collected from patients with candida vulvovagi-nitis which were primarily identified to be Candida by microscopic examination;the fungi DNA was extracted directly from the samples and ITS1-2 region was amplified, and the strains were decided after sequence;the identified results were compared with the culture colo-nies. Results The sequence successful rate of the direct extract from the secretion was 94%（47/50） while that after SDA culture was 64%（32/50）, the difference was of statistical significance. Conclusions It can provide fast identification of the common pathogenic fun-gi to make PCR amplification to the directly extracted DNA from vaginal secretion and then do the sequence, which is faster and more accurate than the traditional culture.