活性炭和不同碳源对烟草苯乙酸一步成苗培养的影响

The Effect of Active Carbon and Series of Carbonhydrates Used in Tobacco on Haploid Plantlet Somatic Tissue PAA One-Step Seedling Formation Culture

通过将生长素苯乙酸(PAA)应用于烟草单倍体植株叶脉组织培养,研究了活性炭和不同碳源在烟草单倍体植株叶脉组织苯乙酸(PAA)一步成苗培养中的作用及机理。结果表明,麦芽糖并不是促使PAA一步成苗培养成功的唯一碳源,葡萄糖具有同麦芽糖相同的功效。添加活性炭后,蔗糖也可促进PAA一步成苗的发生,主要机理是活性炭吸附了培养基中的5-羟甲基糠醛而促使PAA一步成苗的发生。果糖(或蔗糖分解出果糖)不是一步成苗的直接抑制因子,只是在高温高压灭菌时易分解成5-羟甲基糠醛的前体物质,一定浓度的5-羟甲基糠醛是PAA一步成苗的直接抑制因子。试验还表明,PAA对一步成苗的促进效应与碳源的种类和活性炭的添加与否有关。

By applying the auxin phenylacetic acid （PAA） in tobacco haploid plantlet leaf vein tissue somatic culture ,the actionand mechanism of avtive carbon and different category of the carbonhydrate effect PAA used in leaf vein tissue culture one-stepseedling formation of tobacco haploid plantlet was researched. The results showed that maltose is not the sole carbon sourceprompting PAA one-step seedling formation success, the glucose have the same effect with the maltose. After adding activatedcarbon, sucrose may also promote the occurrence of PAA one-step seedling formation.the reason was proved by the experiment thatsucrose will be decomposed into fructose and glucose in the condition of high-press- -killing-bacterium. And, in the same condition,fructose can be decomposed into 5-hydroxymethyl furfural （HMF）. HMF is an inhibitor of one-step seedling formation which can beabsorbed by avtive carbon. The experiments show that the effect of PAA stimulating one-step seedling formation appears to dependon the category of the carbonhydrate and active carbon.