黄连解毒汤对脑梗塞急性期大鼠胃肠动力的调节作用

Regulatory Effect of Huanglian Jiedu Tang(黄连解毒汤) on Rats' Gastrointestinal Motility in Acute Stage of Cerebral Infarction

目的:研究黄连解毒汤对脑梗塞急性期大鼠胃动素(MTL)及血管活性肠肽(VIP)的影响。方法:制备大鼠大脑中动脉梗塞模型,随机分为脑梗塞组、黄连解毒汤常规剂量组、黄连解毒汤高剂量组、西咪替丁组、假手术组,并设正常对照组。分别给予生理盐水、黄连解毒汤常规剂量、黄连解毒汤高剂量、西咪替丁、生理盐水灌胃,分别于4、7天后测定血浆胃动素、血管活性肠肽含量。结果:脑梗塞大鼠4天、7天时MTL、VIP含量较相应正常组、假手术组均有升高(P<0.01),黄连解离汤高剂量组MTL、VIP较模型组明显降低。4天时与黄连解毒汤常规剂量组相比,黄连解毒汤高剂量组MTL水平具有显著统计学差异(P<0.01),而VIP水平也具有统计学意义(P<0.05);黄连解毒汤常规7天组MTL、VIP含量较4天组降低(P<0.05)。结论:黄连解毒汤能有效调节脑梗塞急性期大鼠胃动素及血管活性肠肽的表达,且疗效与剂量有关。

Objective： To observe the effect of Huanglian jiedu decoction（ HLJDT） on the changes of the motilin（ MTL） and vasoactive intestinal peptide（ VIP） in the acute stage of cerebral infarction rats. Methods：Models of middle cerebral artery occlusion（ MCAo） as cerebral infarction were made,they were divided into cerebral infarction group,HLJDT routine dose group,high dose group,cimetidine group,sham operation group randomly,another rats were chosen as the normal control group. Rats were given sodiun chloride,HLJDT routine dose,HLJDT high dose,cimetidine,sodiun chloride respectively. On the forth day and the seventh day,inferior vena venous blood samples were collected to detect MTL and VIP concentrations by radioimmunoassay. Results：On the 4th and 7th day,plasma MTL and VIP of cerebral infarction group and still higher than those of the normal group and sham operation group,those in HLJDT high dose group were lowered obviously（ P〈0. 01）; on the 4th day,compared with HLJDT routine dose group,the MTL level of HLJDT high dose group has significant difference（ P〈0. 01）,and there was also significant in level of VIP（ P〈0. 05）; The 7th day,MTL and VIP of HLJDT routine dose group were lower than those on the 4th day（ P〈0. 05）. Concultion： HLJDT can effectively regulate the expression of MTL and VIP,and the efficacy were dose- related.