染料木素对2型糖尿病心肌病大鼠心肌细胞纤维化的影响

Effects of Genistein on Myocardial Fibrosis in Type 2 Diabetic Cardiomyopathy Rats

目的:探讨染料木素(Gen)对2型糖尿病心肌细胞纤维化的抑制或修复作用。方法:取8只SD大鼠作为正常对照组;取以高脂饲料且注射链脲佐菌素后造模成功的32只糖尿病大鼠并随机均分成糖尿病心肌病模型组、二甲双胍对照组、染料木素低剂量干预组、染料木素高剂量干预组等4组;从第7周起每组大鼠均以普通块料喂养,同时,在第11周测定血糖水平等指标后,将各组大鼠处死后迅速取出心脏,经脱水、浸蜡、切片后进行HE染色,光镜观察心肌组织学变化,免疫组化染色观察TGF-β1表达。结果:Gen可明显降低模型大鼠血糖,改善心肌病理学变化,降低TGF-β1阳性表达。结论:高剂量Gen对2型糖尿病导致的心肌纤维化具有显著的修复作用。

Objective： To prove whether Gen can restrain or restore the myocardial fibrosis caused by type 2diabetes or not. Methods： 8 SD normal rats were chosen as the natural control group,while the other 32 SD diabetes rats were averagely separated into 4 groups as model,MET,Gen- L and Gen- H which were achieved successfully by feeding high fat foods and injected with streptozocin. From the seventh week,in addition to ordinary food,Gen was taken orally in amount of 30 mg / kg body weight for cen- L and 60 mg / kg body weight for the Gen- H,MET was taken orally in amount 4. 2 mg / kg body weight,the normal saline was taken for the control and the model regularly without any other disposal. All rats were handled to death after the sugar level was measured in the eleventh week,then the hearts were disposed through a series of procedures such as dehydration,paraffin,slicing,HE stain,immunohistochemistry staining. At length,all the sections were observed and analyzed through the optical microscope. Results： Gen could reduce blood sugar of rats,improve the pathologic changes of diabetic cardiomyopathy,decrease the positive expression of TGF- β1. Conclusion： High dosage gen had the distinct restoring effect on the myocardial fibrosis which resulted from the type 2 diabetes mellitus.