摘要
目的:探讨游泳运动对攻击行为大鼠海马神经元5-HT1A-R、5-HT1B-R蛋白水平的表达变化,为运动改善大鼠攻击行为发生机制的阐明提供实验依据。方法:成年健康雄性SD大鼠36只,适应1周后随机分为:安静对照组、攻击模型组、攻击模型游泳组、入侵组。通过旷场实验检测大鼠的行为活动,采用Western-blot法及免疫组织化学法检测大鼠海马神经元5-HT1A受体、5-HT1B受体蛋白表达变化。结果:攻击模型建立成功后大鼠的总路程、平均速度、中央活动时间均较高,经过8周游泳运动干预后攻击模型大鼠的总路程、平均速度、中央活动时间均下降P<0.05;攻击模型组大鼠海马神经元5-HT1A受体、5-HT1B受体蛋白表达明显下降(P<0.05),经过8周游泳运动干预后大鼠海马5-HT1A受体、5-HT1B受体蛋白表达明显升高(P<0.05)。结论:游泳运动对攻击模型大鼠攻击行为具有改善作用,其作用机制可能与海马神经元5-HT1A受体、5-HT1B受体表达增强,使5-HT能系统功能提高,从而增强了中枢神经抑制作用有关。
Objective:The purpose of this study was to test the effect of swimming on hippocampus neurons 5-HT1A-R and 5-HT1B-R protein levels in rats with aggressive behavior,for providing references to explain the mechanism of exercise alleviating aggressive behavior.Methods:Thirty-six SD male rats were divided into following four groups after one-week adapted training,groups are:control group(C),aggressive behavior group(A),aggressive behavior and swimming group(AS),and invasion group(I).Rats' activity was tested by field experiment,hippocampus neurons 5-HT1A-R and 5-HT1B-R protein levels were measured by Western blotting and Immunohistochemistry.Results:The total distance,mean speed and central activity time in A group were higher than those in C group,but they were decreased in AS group(P 0.05) compared to A group.5-HT1A-R and 5-HT1B-R protein levels were lower in A group than those in C group(P 0.05),but they were increased in AS group compared to A group(P 0.05).Conclusion:Swimming can alleviate rats' aggressive behavior that is related to the increase of 5-HT1A-R and 5-HT1B-R after swimming that improves 5-HT system and enhances the inhibition effect of central nervous.
出处
《北京体育大学学报》
CSSCI
北大核心
2015年第1期68-73,共6页
Journal of Beijing Sport University
基金
陕西师范大学"211工程"学科建设项目(2014)