rhGM-CSF、纳米银外用对深Ⅱ°烫伤创面愈合的影响研究

Effects of recombinant human Granulocyte/ macrophage colonystimulating factor and nanosilver for deep burn degreen Ⅱ wound healing

目的 :研究外用重组人粒细胞-巨噬细胞集落刺激因子(rh GM-CSF)和纳米银,对深Ⅱ°烫伤创面愈合的影响。方法 :用Wistar大鼠建立深Ⅱ°烫伤模型,分为A、B、C 3组,A组(30例):凡士林纱布覆盖;B组(30例):纳米银覆盖;C组(30例):rh GM-CSF涂抹创面;伤后第1、4、7、10、14、21天,观察创面愈合情况,计算愈合率,按照酶联免疫吸附法测定血清中血管内皮生长因子(VEGF)及表皮生长因子(EGF)水平。结果:A、B、C 3组均在伤后第10天出现血管化和上皮化;创面愈合率C组>B组>A组,第10天各组之间差异无统计学意义(P>0.05),第14、21天各组之间差异有统计学意义(P<0.05);VEGF水平,A组、B组、C组分别在第21、14天达峰值,伤后第1天各组间差异无统计学意义(P>0.05),第4、7、10、14天各组间及第21天A组和C组之间差异有统计学意义(P<0.05);EGF水平,各组均在第21天达峰值,第1、4天各组间及第7天A组与B组间差异无统计学意义(P>0.05),第7天C组与A组、B组之间及第10、14、21天各组间差异均有统计学意义(P<0.05)。结论:rh GM-CSF、纳米银外用,均加速深Ⅱ°烫伤创面愈合,并且rh GM-CSF效果优于纳米银。

Objective：To observe the effect of recombinant human Granulocyte/macrophage colonystimulating factor（rhGM-CSF） and nano-silver as treatment for deep burn degreen ]] wound healing. Methods：The burn models were done with Wistar rats, which were randomly divided into three groups, petrolatum treatment （group A. n = 30）,nano-silver treatment （group B. n = 30）,and rhGM- C SF treatment（group C. n = 30）. The healing rate of the three groups was recorded on postburn day 1,4,7, 10, 14,21. Meanwhile the levels of VEGF and EGF in serums were measured with ELISA. Results：Group A,group B,and group C were all ueovascularization and epithelization on postburn day 10. The healing rate：group C〉group B〉group A. On postburn day 10,there were no significantly difference between groups（P 〉 0.05）,but on postburn day 14,21, there were significantly statistical differences between groups （all P 〈 0.05）. For VEGF levels,group A,group B,and group C were peaked on postburn day 21,14, 14,respectively. On postburn day l, there were no significantly statistical difference between groups（P 〉 0.05）.On postburn day 4,7, 10,14 and 21 ,there were significantly statistical ditference between groups（P 〈 0.05）. For EGF levels, All groups were peaked on postburn day 21. On postbum day 1 and 4,there were no significantly statistical differences between groups（P 〉 0.05）. On postburn day 7 there wrer ditt^rences between C and group A and group B,and on day 10, 14,21 differences were observed between groups （P 〈 0.05）. Conclusions： rhGM-CSF and nano-silver treatment could promote wound healing, but rhGM-CSF is better than nano-silver.