摘要
为考察鳊鱼(Parabramis pekinensis)幼鱼的匀加速游泳(Constant acceleration swimming)能力,将32尾实验鱼随机均分为4组,即对照组和3个处理组(每组样本量n=8),驯养温度为(25±1)℃。处理组分别在0.1、0.2和0.4cm·s-2的加速度(a)条件下,测定实验鱼的最大匀加速游泳速度(Constant acceleration speed,Ucat)及游泳测定过程历时(t);游泳力竭后,立即取血浆、白肌和肝脏,进行生化测定(对照组不经游泳直接取样进行生化测定)。实验结果显示:随着a的增大Ucat分别为62.85、73.66、75.62cm·s-1,其中0.2cm·s-2组及0.4cm·s-2组的Ucat无显著差异,但均显著大于0.1cm·s-2组(p<0.05);t显著下降(p<0.05),分别为:632、349、174s;肌乳酸含量和血乳酸浓度增大,当a为0.4cm·s-2时,肌乳酸含量和血乳酸浓度均显著高于对照组(p<0.05);4组之间的肝乳酸含量无显著差异;处理组的肌葡萄糖含量随着a的增大而显著增加(p<0.05),但0.1cm·s-2组的肌葡萄糖含量显著低于对照组(p<0.05);4组之间的肌糖原含量无显著差异。研究表明随着a的增大,鳊鱼幼鱼的Ucat增加,t缩短,肌乳酸含量增大,这应与鱼类无氧代谢参与程度的增加密切相关;血乳酸水平的提高应由"乳酸泄露"所致;而底物消耗与匀加速游泳能力及游泳路程有关。
To investigate the constant acceleration swimming performance of juvenile Chinese bream (Parabramis pekinensis), 32 Chinese bream juveniles were selected and divided into 4 groups (control group, n=8; treatment groups, n=3 × 8), and reared at (25± 1) ℃. Then the constant acceleration speed (Ucat) and the duration of measurement process (t) of experiment fish under ac- celeration of 0. 1, 0. 2, 0. 4 cra · s-1 were measured. Then, the plasma, white muscle and liver were sampled for biochemical indi- cator measurements immediately after Uc, t tests (the fish in control group were sampled without swimming). The Ucar were 62.85, 73. 66 and 75.62 cm · s-1 in 0. 1, 0. 2, 0.4 cm · s-2 acceleration groups. There was no significant difference in Ucar between 0. 2 and 0.4 cm · s-2 acceleration groups, but U~,t of both groups were significantly higher than that of 0.1 cm · s-2 acceleration group (p〈 0.05). The t decreased significantly with the increase of acceleration rate (p〈0.05), and the values were 632, 349 and 174 s, re- spectively, in 0. 1, 0.2, 0.4 cm · s-2 acceleration groups. The white muscle lactate content ([lactate]) and plasma [-lactate] in- creased with the increase of acceleration. The white muscle [lactate] and plasma [lactate】 were significant higher than that of con- trol group when acceleration rate was 0.4 cm · s-2. There were no significant difference in liver [lactate] among control and three treatment groups. The white muscle [glucose] of treatment groups significantly increased with the increase of acceleration. Whereas white muscle [glucose] of 0. 1 cm· s-2 was significantly lower than that of control group (p〈0.05). There were no significant differences in white muscle [glycogen] among control and treatment groups. The results show that the Ucar and white muscle [lac- tate] increased, but t became shorter with the increase of acceleration rate possibly due to the increased recruitment of anaerobic me- tabolism. The plasma [lactate] increased after UCat test due to the release of muscle lactate. The acceleration swimming capacity hence swimming distance was related to the substrate consumption in Chinese bream juvenile.
出处
《重庆师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2015年第2期13-17,共5页
Journal of Chongqing Normal University:Natural Science
基金
国家自然科学基金(No.31172096)
重庆科技创新能力建设项目(No.CSTC2010CA1010)