HPLC法同时测定六味能消胶囊中木香烃内酯、去氢木香烃内酯大黄素、大黄酚的含量

Simultaneous determination of costunolide,dehydrocostus lactone,emodin and chrysophanol in Liuweinengxiao capsules by HPLC

目的：建立六味能消胶囊中木香烃内酯、去氢木香烃内酯、大黄素、大黄酚4个成分含量测定的方法。方法：采用Atlantis C18色谱柱（150 mm×4.6 mm,5μm）,以甲醇-0.1%磷酸水为流动相,梯度洗脱,检测波长225 nm（0～25 min检测木香烃内酯、去氢木香烃内酯）、254 nm（25～40 min检测大黄素、大黄酚）,柱温25℃。结果：木香烃内酯、去氢木香烃内酯、大黄素、大黄酚的线性范围分别为0.665 6～33.28、0.563 7～31.84、0.524 8～26.24、0.585 6～29.28μg·mL^-1;平均回收率（n=6）分别为99.28%（RSD=1.8%）,101.7%（RSD=1.6%）,101.2%（RSD=2.0%）,99.36%（RSD=1.8%）。5批样品中木香烃内酯、去氢木香烃内酯、大黄素、大黄酚含量分别为2.32～2.41、2.48～2.62、0.42～0.48、1.26～1.33 mg·g^-1。结论：该方法可用于六味能消胶囊中木香烃内酯、去氢木香烃内酯、大黄素、大黄酚的同时测定。

Objective： To develop an HPLC method for the determination of costunolide,dehydrocostus lactone,emodin,and chrysophanol in Liuweinengxiao capsules. Methods： Samples were analyzed on an Atlantis C18column（ 150 mm × 4. 6 mm,5 μm） eluted with methanol and water containing 0. 1 % phosphate acid as mobile phases in a linear gradient mode. The flow rate was kept at 1. 0 m L·min^-1,and the column temperature was set at 25 ℃. The detector wavelengths were 225 nm（ 0-25 min） for costunolide and dehydrocostus lactone,254 nm（ 25-40 min） for emodin and chrysophanol. Results： The linear ranges of costunolide,dehydrocostus lactone,emodin and chrysophanol were 0. 665 6-33. 28 μg·mL^-1,0. 563 7-31. 84 μg·mL^-1,0. 524 8-26. 24 μg·mL^-1,0. 585 6-29. 28 μg·mL^-1,respectively. The average recoveries（ n = 6） of the four constituents were 99. 28 %（ RSD = 1. 8 %）,101. 7 %（ RSD = 1. 6 %）,101. 2 %（ RSD = 2. 0 %）,99. 36 %（ RSD = 1. 8%）,respectively. The contents of the four constituents were 2. 32-2. 41 mg·g^-1,2. 48-2. 62mg·g^-1,0. 42-0. 48 mg·g^-1,and 1. 26-1. 33 mg·g^-1. Conclusion： The established method can be used for simultaneous determination of costunolide,dehydrocostus lactone,emodin and chrysophanol in Liuweinengxiao capsules.