蛇葡萄素纳米胶束含量与包封率的测定

Determination of content and entrapment efficiency of ampelopsin nanomicelles

目的：建立蛇葡萄素纳米胶束含量与包封率的测定方法。方法：采用薄膜分散法制备蛇葡萄素纳米胶束,以反透析法分离纳米胶束和游离药物,建立HPLC法测定蛇葡萄素含量。色谱条件：采用Wondasil C18（4.6 mm×250 mm,5μm）色谱柱,以甲醇-水-磷酸（35∶65∶0.2）为流动相,流速1.0 m L·min^-1,检测波长292 nm,柱温25℃,进样量20μL。结果：蛇葡萄素质量浓度在10～300μg·mL^-1范围内与峰面积呈良好的线性关系（r=0.999 5）,回收率为99.3%～101.3%,日内及日间精密度均小于2%。反透析法能将纳米胶束与游离药物良好地分离,游离药物回收率为（99.1±1.7）%,加样回收率为（97.7±1.4）%,平均包封率为（80.4±1.0）%。结论：该法简单易行、结果准确,可用于蛇葡萄素纳米胶束的含量与包封率测定。

Objective： To establish a method for the determination of content and entrapment efficiency of ampelopsin nanomicelles. Methods： Ampelopsin nanomicelle was prepared with film dispersion method. The retrodialysis method was used to separate the free drug from the nanomicelles and the content of ampelopsin nanomicelles was determined by HPLC with the Wondasil-C18column（ 4. 6 mm × 250 mm,5 μm）,the mobile phase was methanol-water-phosphoric acid（ 35 ∶ 65 ∶ 0. 2） at a flow rate of 1. 0 m L·min^-1,the detection wavelength was292 nm,the column temperature was 25 ℃ and the injection volume was 20 μL. Results： A good linear relationship was found between the peak area and the concentration of ampelopsin ranging from 10 μg·mL^-1 to 300 μg·mL^-1（ r = 0. 999 5）. The recovery of ampelopsin was in a range of 99. 3%-101. 3%. Intra-day and inter-day RSDs were less than 2%. The free drug was well separated from the nanomicelles by retrodialysis method. The free drug recovery was（ 99. 1 ± 1. 7） %. The sample recovery was（ 97. 7 ± 1. 4） %. The average entrapment efficiency of ampelopsin nanomicelles was（ 80. 4 ± 1. 0） %. Conclusion： The proposed method is simple,applicable and accurate for determination of content and entrapment efficiency of ampelopsin nanomicelles.