摘要
以文冠果的花粉为试材,研究不同培养基及贮藏条件对文冠果花粉生活力的影响。结果表明：培养基中硼酸对花粉萌发的影响最大,蔗糖次之,琼脂最小;适合文冠果花粉萌发的培养基中蔗糖浓度为10%~15%,硼酸浓度为0.01%~0.05%,琼脂浓度为0.5%~0.6%;贮藏条件对文冠果花粉生活力有很大影响,-20℃冷冻条件下有利于保持花粉的生活力,4℃低温贮藏次之,25℃条件不利于花粉的长期贮藏。该研究可为文冠果生产上的花粉贮藏和人工授粉提供参考依据。
Taking pollen of Xanthoceras sorbiJblia as material,the pollen viability of Xanthoceras sorbifolia under the different culture media and the storage conditions was studied. The results showed that, the effect of boric acid on pollen germination was the greatest in the culture medium,followed by sucrose and agar. The suitable sucrose concentration to pollen viability was 10% ~ 15% in the culture medium, boric acid concentration was 0. 01% ~ 0. 05%, and agar concentration was 0. 5% ~0. 65. Storage temperature had great effect on pollen viability. --20℃ was favorable for keeping pollen viability,and 4℃ took the second place,and 25℃ was not conducive to the pollen storage. So reference basis for pollen-storage and artificial pollination of Xanthoceras sorbifolia in the production was offered.
出处
《北方园艺》
CAS
北大核心
2015年第5期64-67,共4页
Northern Horticulture
基金
沈阳农业大学"林业种质创新与培育"学术创新团队资助项目(2013-4-B-2)
关键词
文冠果
花粉生活力
培养基
贮藏条件
Xanthoceras sorbifolia
pollen viability
culture medium
storage condition
