摘要
以番茄高酸品种"12680"为母本,低酸品种"12574"为父本,配制杂交组合,构建含有260个单株的F2代群体为试材,采用酸碱滴定法测量果实的总酸含量,并进行遗传图谱构建及QTL分析,研究番茄果实中有机酸含量的遗传规律并找到与有机酸含量相关的QTL位点。结果表明:在256对AFLP引物和185对SSR引物中获得多态性良好的AFLP引物24对,SSR引物17对;构建连锁图谱,该图谱包括6个连锁群,共42个标记,覆盖基因组长度1 358.93cM,检测LOD值大于2.5的标记2个,贡献率分别为20.27%、10.98%,均分布在第2连锁群上。其中Ta1为负向加性效应,Ta2为正向加性效应。
An AFLP and SSR genetic linkage map was constructed in tomato with a 260 intraspecific Fz population derived from a cross between high acid content line ＇12680＇ as female parent and low acid contend line ＇ 12574＇ as male parent. Total acid content was determined by aeid-base titration,total organic acid genetic development was studied to find QTL position related to organic acid ranted. The results showed that, in totally chose 256 paris of AFLP molecule markers, 185 paris of SSR molecule markers, 24 paris of AFLP molecule markers and 17 paris of SSR molecule markers had favorable polymorphism. F2 generation were analyzed for patterns to construct the six linkage groups. It contained 41 molecule markers,the total length of 1 358. 93 cM. There were two markers detected the LOD scores greater than 2. 5,genetic contribution rate were 20. 27% and 10. 98%. The 2 QTLs distributed in 2 linkage groups,among which Ta 1 was negative additive effects, Ta 2 was positive additive effect.
出处
《北方园艺》
CAS
北大核心
2015年第5期109-113,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(NSFC 31272171)
