陕甘两省花椒疫霉病病原鉴定及其拮抗菌筛选

Identification of PhytophthoraSpecies Causing Pricklyash Blight from Shaanxi and Gansu Provinces and Screening of Antagonistic Actinomycetes

以采自陕西、甘肃的花椒疫霉病菌为试材,依据病原菌的菌落与繁殖体特征、rDNA-ITS及β?tubulin基因序列分析和致病性测定,研究该病菌的种类与分布,并对花椒疫霉菌拮抗菌进行了筛选。结果表明:陕西宝鸡地区的疫霉菌菌系Pfs3、Pfs4、Pbjt和Pfs5为苎麻疫霉(Phytophthora boehmeriae Sawada);甘肃陇南地区菌系Pcsx、Pwmc、Pwqh、Pwbs3和Pwbs2为多寄主疫霉(Phytophthora multivora Scott&Jung),从花椒园土壤中分离筛选出1株对疫霉菌具有拮抗效果的放线菌zy-1,初步鉴定为链霉菌之一种(Streptomyces sp.)。

Taking species and distribution of Phytophthora which were isolated from pricklyash （Zanthoxylurn bungeanum） in Shaanxi and Gansu provices as material, based on colony,sporangium and oogonium morphology, ITS and β-tubulin gene sequences and their pathogenicity,species and distribution of the bacteria were studied,and the antagonistic bacteria of pricklyash Phytophthora were screened. The results showed that Phytophthora isolates Pfs3, Pfs4, Pbjt and Pfs5 from tMoji of Shaanxi were identified as Phytophthora boehmeriae Sawada, and isolates Pcsx, Pwmc, Pzoqh, Pwbs3 and Pzabs2 from Longnan of Gansu were identified as Phytophthora multivora Scott and Jung. One actinomycetes isolated from the soil of prieklyash field was screened out, and preliminary identified as Streptomyces sp. which showed antagonistic activity to these Phytophthora species.