摘要
以南昌郊蛟桥镇甜瓜基地的甜瓜炭疽病病果为试材,采用致病性测定、形态学观察和分子生物学的方法,研究了甜瓜炭疽病病原归属。结果表明:黄瓜炭疽病病原菌形态具典型炭疽菌属类真菌Colletorichum orbiculare的特征,GPDH基因序列与NCBI-GenBank中已有的C.orbiculare序列同源性为100%,接种发病症状与自然发病症状类似,因此将该病原菌鉴定为瓜类刺盘孢(Colletorichum orbiculare)。
Taking the disease melon of anthracnose from Jiaoqiao town in Nanchang for test material, the pathogen was identified by the study of morphology,pathogenicity,cultural feature and the sequence of GPDH. The results showed that the sequence of GPDH of isolate NCBI showed a sequence identity of 100% with C. orbiculare published in GentBank. The vaccination symptoms was similar to the filed symptoms, so the fungus was identified as C. orbiculare.
出处
《北方园艺》
CAS
北大核心
2015年第5期132-134,共3页
Northern Horticulture
基金
江西省教育厅科学技术研究资助项目(GJJ13269)
关键词
甜瓜
炭疽病
病原鉴定
瓜类刺盘孢
melon(Cucumis melo L.)
anthrancose
identification
Colletorichum orbiculare