肺癌细胞微生态成分分析及其在耐药性方面的作用及机制

Microecological components of lung cancer cells and their relationship with and mechanism of drug resistance

目的探析肺癌细胞微生态环境中蛋白成分及其与人肺癌多药耐药细胞系SPC-A1/TAX耐药性之间的关系。方法通过基因芯片、蛋白质谱实验并借助S-P法以及MTS法来分析蛋白与耐药之间的关系。结果 SPC-A1/TAX与SPC-A1相比,除了对紫杉醇具有耐受性外,对其他抗肿瘤药物也有一定程度的耐药性。SPC-A1中,GST-π以及MRP的阳性率均为0,而SPC-A1/TAX中两者的阳性率均为100%,对其他抗肿瘤药物也有一定程度的耐药性,耐药倍数分别为6.34、2.42、13.51、2.65、9.80、7.00。结论多药耐药相关蛋白及谷胱甘肽-S-转移酶π与人肺癌多药耐药细胞系SPC-A1/TAX的耐药性密切相关,是导致其具有耐药性的重要机制。

Objective To analyze the multi-resistance related proteins and GSH-S-transferase PI in lung cancer and their relationship with the drug resistance of lung cancer multidrug resistant cell line SPC A1 / TAX. Methods Genechip experiment,S-P method and MTS method were performed to analyze the relationship between the proteins and the drug resistance. Results Compared with SPC-A1,SPC A1 / TAX had not only tolerance to paclitaxel,but also certain degree of resistance to other antineoplastic drugs. The positivity rates of both GST-PI and MRP in SPCA1 were 0,while those in SPC A1 / TAX were 100%. The resistance ratios to the tested antitumor drugs were6. 34,2. 42,13. 51,2. 65,9. 80 and 7. 00,respectively. Conclusion The multi-resistance related proteins and GSH-S-transferase PI in lung cancer multidrug resistant cell line SPC A1 / TAX is closely related to the resistance of lung cancer,which is an important mechanism of its drug resistance.