响应面法优化植物乳杆菌产β-半乳糖苷酶发酵培养基

Optimization of medium forβ-galactosidase production from Lactobacillus plantarum by response suface methodology

采用单因素试验分析不同碳源、氮源对植物乳杆菌KLDS1.0628产β-半乳糖苷酶的影响,在此基础上利用Plackett-Burman,Box-Behnken实验设计对发酵培养基成分含量进行优化。结果表明,Plackett-Burman设计筛选出3个主要因素为乳糖、乙酸钠、柠檬酸氢二铵,其最佳质量浓度分别为16.41,10.21,5.83 g/L。在优化后的培养基下,β-半乳糖苷酶活可达3.32 U/m L,与理论预测值3.40 U/m L基本一致,酶活比优化前提高了73.8%。

Different carbon sources and nitrogen source were analyzed by using single factor analysis method. Based on this, the optimized medium was obtained by Plackett-Burman, Box-Behnken design. The results indicated that lactose、sodium acetate、diammonium hydrogen citrate were proved be the important factors in the medium by PB design,and the optimal concentrations of the variables were determined as：lactose 16.41 g/L, sodium acetate 10.21 g/L, diammonium hydrogen citrate 5.83 g/L. Under the optimal culture condition,theβ-galactosidase activity was up to 3.32 U/m L, that was consistent with the maximum theoretic value 3.40 U/m L, the activity ofβ-galactosidase was increased by 73.8% compared with the previous condition.