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表没食子儿茶素没食子酸酯抑制脂多糖诱导巨噬细胞趋化因子的表达 被引量：2

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摘要 目的探讨表没食子儿茶素没食子酸酯(EGCG)对脂多糖(LPS)诱导巨噬细胞趋化因子单核细胞趋化蛋白1(MCP-1)和巨噬细胞炎症蛋白2(MIP-2)表达的影响。方法小鼠RAW264.7巨噬细胞用1 mg/L LPS进行刺激,采用荧光实时定量PCR(qRT-PCR)检测MCP-1与MIP-2 mRNA水平、ELISA检测培养液中MCP-1与MIP-2的蛋白含量。结果 1 mg/L LPS可显著刺激RAW264.7细胞MCP-1和MIP-2 mRNA表达,单独EGCG对MCP-1和MIP-2基因表达无明显影响,但EGCG可以抑制LPS诱导的MCP-1和MIP-2的表达,并存在剂量依赖效应。结论 EGCG能以剂量依赖方式抑制LPS诱导的巨噬细胞MCP-1和MIP-2的表达。
作者 张东芳 崔海燕 肖鹏 杨欢 李卫国 王坤英
机构地区 河南师范大学生命科学学院
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2015年第1期58-61,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 河南省重点科技攻关计划(122102310282) 河南省教育厅自然科学基础研究计划(2010A180011)
关键词 表没食子儿茶素没食子酸酯 脂多糖 巨噬细胞 单核细胞趋化蛋白-1 巨噬细胞炎症蛋白-2
分类号 Q786 [生物学—分子生物学] R392.11 [医药卫生—免疫学]
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共引文献20

同被引文献37

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细胞与分子免疫学杂志
2015年 第1期

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