PAPP-A、PLGF、sFlt-1预测妊娠期高血压疾病发病风险价值的研究

The valuable of PAPP-A,PLGF and sFlt-1 in predict the risk of hypertensive disorders in pregnancy

目的探讨妊娠相关蛋白A(PAPP-A)、胎盘生长因子(PLGF)、可溶性血管内皮生长因子(sFlt-1)在预测妊娠期高血压疾病发病风险中的价值。方法采用酶联免疫法(ELISA)检测66例孕妇不同孕周的血清PAPP-A、PLGF和sFlt-1水平。其妊娠期高血压组33例(观察组),正常妊娠组33例。采用荧光定量PCR及Western blot方法分别检测胎盘组织中PLGF、sFlt-1 mRNA及蛋白表达。结果在孕程的全部采血孕周,观察组孕妇血清sFlt-1水平均明显高于对照组,而PLGF均低于对照组。观察组各孕周内sFlt-1/PLGF均显著高于对照组。观察组胎盘组织sFlt-1 mRNA及蛋白表达水平明显高于对照组;PLGF mRNA及蛋白表达水平明显低于对照组。结论孕妇血清中PAPP-A、PLGF、sFlt-1水平变化能够作为诊断和预测妊娠期高血压疾病的临床客观指标。

Objective To explore the valuable of PAPP- A,PLGF and sFlt-1 in predict the risk of hypertention disorder complicating pregnancy（ HDCP）. Methods Thirty- three patients with hypertention disorder complicating pregnancy and thirty- three normal pregnant women were enrolled. The mRNA expression of sFlt-1 and Pl GF in placenta was determined by Realtime- PCR and western blot. The levels of PAPP- A,Pl GF and sFlt-1 in serum were determined by enzyme- linked immunosorbent assay（ ELISA）. Results The mRNA expression of Pl GF in placentaand the mean concentration of Pl GF in serum of preeclampsia patients were significantly lower than those in normal groups. The mRNA and protein of sFlt-1 in placenta and the mean concentration of sFlt-1 in serum of HDCP patients were also significantly higher than those in normal groups. The PAPP- A in serum of HDCP patients were also significantly higher than those in normal groups. Conclusion The PAPP-A,PLGF and sFlt-1 levels in maternal serum can be used as the clinical objective indicators to diagnostic and prognostic hypertensive disorders in pregnancy.