肿瘤干细胞标志物CD133和Nestin在卵巢癌中的表达及意义

The expression and significance of CD133 and nestin as the tumor stem cell markers in ovarian cancer

目的探讨肿瘤干细胞标志物CD133和Nestin在卵巢癌中的表达,并研究其与临床病理参数及预后的相关性。方法采用免疫组化法检测20例正常卵巢组织与50例上皮性卵巢癌组织,检测其中CD133及Nestin的表达水平,并分析其与临床病理因素之间的关系。结果 1CD133和Nestin在正常卵巢组织和卵巢上皮性癌中的阳性表达率分别为10.0%、62.0%及10.0%、76.0%（P〈0.05）。2CD133及Nestin在卵巢癌组中的表达水平与其FIGO分期及病理分级有关,在Ⅲ～Ⅳ期、低分化组（G3）比Ⅰ～Ⅱ期、高中分化（G1、G2）组表达率高（P〈0.05）。3Spearman相关性分析显示CD133表达与Nestin的表达呈正相关（r=0.363,P〈0.05）。结论 CD133与Nestin在卵巢上皮性癌中高表达,可能促进了卵巢上皮癌的发生、发展,并可能作为卵巢癌干细胞标志物用于判断卵巢上皮性癌的预后。

Objective To investigate expression and significance of CD133 and nestin as the tumor stem cell markers in ovarian cancer,and study its correlation with clinicopathological parameters and prognosis.Methods CD133 and nestin protein levels were detected in 50 cases of epithelial ovarian cancer and 20 cases of normal ovarian tissue by immunohistochemistry,and the relationship between their levels and clinical pathological factors was analyzed.Results 1 The positive expression rates of CD133 and nestin in normal ovarian tissue and epithelial ovarian cancer were 10.0%,62.0%,and 10.0%,76.0% respectively（ P0.05）.2The expression levels of CD133 and nestin in ovarian cancer group were associated with FIGO staging and pathologic grading.The expression rate in group III-IV and low differentiation（ G3） was higher than in group I ～ II and high differentiation group（ G1,G2）（ P0.05）.3Spearman correlation analysis showed that CD133 expression was positively correlated to nestin expression（ r = 0.363,P0.05）.Conclusion Over-expression of CD133 and nestin protein may promote the occurrence and development of ovarian epithelial carcinoma,and may be used as ovarian cancer stem cell markers in judging the prognosis of ovarian epithelial carcinoma.