Needle trap-GC-MS法分析云南不同产地薄荷的挥发性成分

Analysis for volatile composition of Mentha haplocalyx Briq. in different regions of Yunnan by Needle trap-GC-MS

对来自云南石林、德宏和西双版纳3个种植地薄荷的挥发性成分进行分析,并探讨其差异,为薄荷的种植和开发利用提供理论依据.应用动态针捕集-气质联用法对3个种植地薄荷的挥发性成分进行分析并探讨动态针捕集-气质联用法在天然植物挥发性成分分析中的适用性.从3个种植地的薄荷中共分离鉴定了44个挥发性成分,主要是单萜和倍半萜萜类及其含氧衍生物,发现石林和德宏种植薄荷的挥发性成分相似度很高,它们L-薄荷醇含量都达70%以上,西双版纳种植的薄荷与二者差别很大,主要成分为百里香酚(20.04%)、香芹酚(19.03%)和对伞花烃(12.19%)等.结果表明不同的生长环境对薄荷的化学组成有很大的影响,石林和德宏种植的薄荷中薄荷醇含量大,适合作为生产薄荷香型香精的香原料,可应用于食品、化妆品和烟草等产业中,而西双版纳种植的薄荷含有很多的药理活性成分,可能更适合应用于传统中草药领域.

Objective： In order to provide a scientific evidence for the cultivation and exploitation of Mentha haplocalyx Briq.,volatile components analysis of peppermint grown in Shilin,Dehong and Xishuangbanna were done.Methods： Volatile components of peppermint in 3 growing areas were analyzed by the Needle trap-GC-MS,and the suitability of using the Needle trap-GC-MS for plant analysis of volatile components was investigated.Results： In total,44 volatile components were separated and identified from peppermint grown in three origins,most of them were monoterpenes and sesquiterpenes terpenoids and their oxygenated derivatives.Those grown in Shilin and Dehong showed a high similarity in volatile components where L-menthol alcohol had a content of more than70%.A big difference was found in the peppermint grown in Xishuangbanna where the main components were thymol（ 20.04%）,carvacrol（ 19.03%） and 4-Isopropyltoluene（ 12.19%）. Conclusions： Different growth environment had a great influence on the chemical compositions of the peppermint.Peppermint that grew in Shilin and Dehong produced high levels of menthol,suitable as a flavor material of producing mint aromas fragrance,which could be used in food,cosmetics and tobacco industries.However,peppermint grown in Xishuangbanna contained a lot of pharmacological active ingredients,might be more suitable for application in traditional Chinese medicine field.