复方黄芪首乌合剂对MS大鼠AngⅡ、FFA分泌及氧化应激反应的作用

The Effects of the Mixture of Radix Astragalus and Polygonum Mulflorum on Renal Secretion of AngⅡ and FFA and Oxidative Stress in Rats with MS

目的:探讨复方黄芪首乌合剂(HQSW)对实验性代谢综合征(MS)大鼠肾组织血管紧张素Ⅱ(AngⅡ)和游离脂肪酸(FFA)分泌及氧化应激反应的影响。方法:采用高糖高脂饲料喂养建立实验性MS大鼠模型,以HQSW(低剂量组、中剂量组和高剂量组)灌胃治疗8周后,测定实验鼠血浆和肾皮质中FFA和AngⅡ浓度,以及丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:MS大鼠血浆和肾组织SOD活力显著下降(P<0.01),而MDA、FFA和AngⅡ水平显著上升(P<0.01)。经中药HQSW干预后,血浆和肾组织SOD活力显著上升(P<0.05);同时MDA、FFA和AngⅡ水平显著下降(P<0.05)。结论:MS大鼠FFA和AngⅡ的分泌异常增多,伴有氧化应激反应异常,经HQSW的干预,上述异常状况得到改善,可能是其防治MS大鼠肾损伤的关键机制之一。

Objective ：To investigate the effect of the mixture of Radix Astragalus and Polygonum mulflorum on renal secretion of angiotensin Ⅱ （Ang Ⅱ） and free fatty acids （FFA） and oxidative stress in rats with metabolic syndrome. Methods：To establish experimental metabolic syndrome rat model through a high - sugar and high - fat diet, Animals were administered with the mixture of Radix Astragalus and Polygonum mulflorum （low dose group, middle dose group and high dose group）. At the end of 8th week, the level of Super Oxygen Dehydrogenises （SOD） and Methane Dicarboxylic Aldehyde （MDA） in the serum and renal cortex were determined by colorimetric method, the level of Ang Ⅱ and FFA in the serum and renal cortex were detected by radioimmunoassay. Resuits：The enzyme activity of SOD in the serum and renal cortex was decreased significantly in rats with MS （P 〈0.01 ）, on the contrary, the level of MDA,AngII and FFA in the serum and renal cortex were increased significantly （ P 〈 0.01 ）. After treated with the mixture of Radix Astragalus and Polygonum mulflorum, The enzyme activity of SOD in the serum and renal cortex was increased significantly （P 〈 0.05 ）, At the same time, The level of MDA,Ang Ⅱ and FFA in the serum and renal cortex were decreased significantly （P 〈 0.05）. Conclusion ：The abnormal secretion of FFA and AngII were occurred in rats with MS, at the same time, the oxidative stress response was being aggravated. After treated with the mixture of Radix Astragalus and Polygonum mulflorum, the above abnormal change was improved, that might be its prevention and treatment mechanism of early renal injury in rats with MS.