中药苦参碱与西药普萘洛尔对心肌梗死后大鼠疗效的对比研究

Comparative Study of the Therapeutic Effect Between Chinese Medicine Matrine and Western Medicine Propranolol on Rat After Myocardial Infarction

目的:研究中药苦参碱与西药普萘洛尔对心肌梗死后大鼠疗效的对比。方法:将60只SD大鼠随机分为6组假手术组、模型组、普萘洛尔5mg/kg剂量组、普萘洛尔20mg/kg剂量组、苦参碱5mg/kg剂量组、苦参碱20mg/kg剂量组。采用左冠状动脉前降支结扎法,制备大鼠急性心肌缺血模型,药物组于术前8小时及术后每日连续灌胃给药,分别于心肌梗死后2天和30天观察Ⅱ导联心电图并分别进行血清肌酸激酶(CK)和乳酸脱氢酶(LDH)活性检测来确定心肌梗死的损伤程度。结果:各剂量组通过与假手术组以及模型组对比,得出普萘洛尔5mg/kg剂量组、普萘洛尔20mg/kg剂量组及苦参碱5mg/kg剂量组、苦参碱20mg/kg剂量组都能降低结扎冠脉后ST段上移、降低心肌缺血大鼠血清中CK和LDH活性,通过2天的数据对比表明普萘洛尔5mg/kg剂量组、普萘洛尔20mg/kg剂量组降低ST段及CK和LDH效果显著(p<0.05);通过30天数据对比表明苦参碱5mg/kg剂量组、苦参碱20mg/kg剂量组降低心电图ST段及CK和LDH效果显著(p<0.05),通过同一药物不同剂量组对比,表明高剂量组作用效果更显著。结论:1.普萘洛尔、苦参碱具有不同程度抗心肌梗死作用;2.抗心肌梗死药物普萘洛尔、苦参碱短期和长期应用心肌缺血后损伤的保护作用各不相同,短期心肌梗死后,普萘洛尔效果较好,长期心肌梗死后,苦参碱对心脏的保护疗效比普萘洛尔要明显;3.通过数据比较,我们认为抗心肌梗死药物的长期应用中,中药苦参碱更适合临床应用。

Objective： This study is aimed at comparing the therapeutic effect between Chinesemedicine matrine and western medicine propranolol on rat after myocardial infarction. Methods 60 SD rats were randomly divided into 6 groups： sham operation group,model group,propranolol- 5mg / kg- dose group,propranolol- 20 mg / kg- dose group,matrine- 5mg / kg- dose group,matrine-20 mg / kg group. By ligation of the left coronary artery before drop method,preparation of acute myocardial ischemia rats,intragastric administration given to drug group 8 hours ahead of the operation and continuously administrated after the operation for days,the lead II ECG and serum creatine kinase（ CK） and lactate dehydrogenase（ LDH） activity assay were respectively observed in order to determine the extent of damage of myocardial infarction in myocardial infarction after 2 days and 30 days. Results： Comparing each dose group with sham operation group and model group,it is concluded that the propranolol 5mg / kg dose group,propranolol 20 mg / kg dose group and matrine 5mg / kg dose group,matrine 20 mg / kg dose group can reduce the ST segment elevation after coronary artery ligation and the myocardial ischemia rats blood CK as well as LDH activity. In addition,the data out of the comparison for 2 days showed that propranolol- 5mg / kg- dose group,propranolol- 20 mg / kg- dose group decreased the ST segment and CK and LDH effect was significant（ p 〈 0. 05）; comparing 30 day- data showed that matrine- 5mg/kg- dose group,matrine- 20mg/kg- dose group decreased ECG ST segment and CK and LDH had significant effect（ p 〈 0. 05）,the effect of Biao Minggao dose was more obvious. Conclusion： 1. propranolol and matrine have different degree effect on anti myocardial infarction; 2. Anti drug propranolol and matrine of short- term and long- term application to myocardial ischemia injury are not identical,but the administration effect of propranolol used in short- term after myocardial infarction is good,and the protection efficacy of matrine used in long term after myocardial infarction on of heart showed more significantly than that of propranolol; 3. it is suggested that in the long- term used drugs of anti myocardial infarction,matrine is more suitable for clinical application.