glypican-3、HepPar1和CD34在肝细胞癌中的表达及临床意义

Expression of glypican-3,Hep Par1 and CD34 in primary hepatocellular carcinoma and their clinical significance

目的探讨glypican-3、Hep Par1和CD34在肝细胞癌(HCC)中的表达及临床意义。方法通过免疫组化方法研究101例肝细胞癌及癌旁组织中glypican-3、Hep Par1和CD34的表达情况,探讨其在肝细胞癌诊断中的意义,并分析其与临床病理特征的关系。结果在HCC中glypican-3阳性率为83.2%,在癌旁正常肝组织内为(-)。在HCC中Hep Par1阳性率为90.1%。glypican-3和Hep Par1的表达与肿瘤分化程度相关(P<0.05),而与患者年龄、性别、微血管浸润、有/无肝硬化均无明显相关性(P>0.05)。CD34呈现特殊的HCC型微血管染色。结论glypican-3、Hep Par1和CD34联合使用对HCC的诊断有更显著的敏感性及特异性,建议作为HCC诊断的首选一线抗体。

Objective To investigate the expression of glypican-3 （ GPC3 ） , HepParl, and CD34 expression in primary hepatocellular carcinoma （ HCC ） and their clinical significance. Methods Immunohistochemistry was used to detect the expression of GPC3, HepParl and CD34 in 101 cases of HCC and adjacent non-cancerous tissues. The diagnostic value of these antibodies in HCC was evaluated. The relationship between their expression and cfinicopathologic characteristics were analyzed. Results The positive expression rate of GPC3 in HCC specimens was 83.2% , and all adjacent non-cancerous tissues were GPC3 negative. The positive expression rate of HepParl in HCC specimens was 90. 1% and all the adjacent non-cancerous tissues were HepPar1-positive. The expression of GPC3 and HepParl was significantly correlated with histological differentiation （ P 〈 0. 05 ）. There were no significant differences between GPC3 and HepParl expression levels in different age group, in male and female groups, vessel invasion and cirrhosis （ P 〉 0. 05 ）. The expression of CD34 was characteristic microvescular staining as HCC type. Conclusion The combined detection of GPC3, Hep Parl and CD34 may be helpful to improve diagnostic sensitivity and specificity of HCC. For HCC, GPC3, Hep Parl and CD34 can be used as the first line antibodies.