海拔对白肋烟质体色素含量及相关基因表达的影响

Effect of Different Altitudes on the Contents of Plastid Pigment and Related Genes Expression of Burley Tobacco

以‘鄂烟1号’为供试品种,于湖北恩施选取高、中、低3个海拔(海拔高度分别为1 560m、1 200m、800m)种植区,研究不同海拔白肋烟主要生育期(旺长期、成熟期)内烟叶质体色素含量及其合成代谢过程中相关基因表达的差异,探讨白肋烟质体色素代谢对海拔高度的生理响应机制。结果表明:烟叶叶绿素a、叶绿素b和叶绿素总量及旺长期类胡萝卜素总量均以中海拔地区最高,且显著或极显著高于其他海拔梯度;成熟期高海拔烟叶类胡萝卜素总量最高,且较旺长期明显增加。叶绿素及旺长期类胡萝卜素合成有关的基因均为中海拔表达最强,成熟期类胡萝卜素合成基因在高海拔表达最强。结果说明‘鄂烟1号’更适宜种植在中海拔地区。

Taking E＇ yan 1 as the material and selecting three altitudes which were the altitude of 1 560 m, 1 200 m and 800 re,respectively,we investigated the difference of the contents of plastid pigment and related genes expression of burley tobacco in Enshi at the fast grow stage and the mature stage. The results showed that the contents of chlorophyll a, chlorophyll b and total chlorophyll in burley tobacco leaves at medium altitude were the highest, and the content of total carotenoids at the fast grow stage among differ- ent altitude had the same trend. The difference between medium altitude and other altitudes was significant or very significant. The leaves of burley tobacco in high altitude had the higher content of total carotenoids at the mature stage and the content of total carotenoids increased fast compared to fast grow stage. In medium altitude planting area, the expression of chlorophyll synthesis genes at two growth stages and carotenoid synthesis genes at fast grow stage were efficient, while the efficient expression of the genes related carotenoid synthesis at mature stage appeared in high altitude planting area. Medium altitude was more suitable for planting E＇ yan 1.