高压氧处理对慢性束缚大鼠行为学和海马区糖皮质激素受体的影响

Effects of hyperbaric oxygen treatment on glucocorticoid receptor expression in the hipopoeampus and on the behavior of rats under chronic restraint stress

目的观察高压氧（HBO）处理和慢性束缚对大鼠行为学及海马区糖皮质激素受体（GR）表达水平的影响，探讨HBO对慢性束缚大鼠的干预作用。方法将60只雄性Wistar大鼠按随机数字表法分成单纯束缚组、单纯HBO组、HBO联合束缚组和空白对照组，每组15只。单纯束缚组给予行为限制3h／d，共21d；单纯HBO组给予2．0ATAHBO治疗每日1次，共21d；HBO联合束缚组，每日先后给予HBO处理和束缚处理，共21d；空白对照组单纯饲养21d，不做任何干预处理。分别于第1天、第11天和第2l天，观察大鼠旷场试验中运动能力（水平得分）及探索行为（垂直得分）的变化，检测第21天海马区GR的表达水平。结果旷场试验显示，干预第11天，单纯束缚组的水平得分[（131．0±20．6）分]和垂直得分[（26．5±4．6）分]均较空白对照组明显升高（P〈0．05）；与单纯束缚组比较，HBO联合束缚组在第1天到第11天的得分升幅减小（P〈0．05）。免疫组织荧光染色显示，单纯束缚组海马区GR表达[（0．2187±0．0184）％]较空白对照组[（0．2203±0．0140）％]显著减少（P〈0．01），HBO联合束缚组与空白对照组比较，差异无明显统计学意义（P〉0．05）。结论慢性束缚导致大鼠行为能力改变和海马区GR表达减少，HBO能够减轻慢性束缚对大鼠行为学和海马区GR表达的影响。

Objective To explore the effects of hyperbaric oxygen treatment on chronic stress and glu- cocorticoid receptor （GR） expression in the hippoeampus. Methods A total of 60 male Wistar rats were ran- domly divided into a restraint group, an HBO （hyperbaric oxygen） group, an HBO-restraint group and a control group using a random number table, each group with 15 animals. All the rats in the restraint and HBO groups were constrained by immobilizing their fore- and hind-limbs on a self-made frame for 3h daily for 21 days, and those in the HBO group received HBO treatment once daily for the same 21 days. The HBO-restraint group was immobilized in the morning and treated with HBO in the afternoon. The control group was reared without any special interven- tion. On the 1st, llth and 21st day of treatment, rats from the different groups were assessed using the open field test. On the 21st day, all the animals were sacrificed and their brains were harvested to detect GR expression. Results In the open field test on the 1 lth day, the restraint group scored （ 131.0 ± 20.6） in terms of motor level and （26.5 ± 4.6） for exploratory behavior, both significantly higher than before restraint and significantly higher than those in the HBO-restraint group at the same time point. Immunofluorescence assay showed that GR expression in the hippocampus of the restraint group was significantly decreased compared with the control group. There was no significant difference, however, between the HBO-restraint group and the control group. Conclusion Chronic re- straint stress induces changes in behavior and GR expression in rats which can be alleviated by hypbaric oxygen treatment.