血浆微RNA-27b-3p作为胃癌早期诊断生物标志物的可行性研究

Feasibility study on plasmatic micro RNA-27b-3p as a potential biomarker for early diagnosis of gastric cancer

目的:探讨血浆微RNA-27b-3p(micro RNA-27b-3p,mi R-27b-3p)作为生物学标志物用于胃癌早期诊断的可行性。方法:采用实时荧光定量PCR法检测46例胃癌患者及40例年龄和性别均匹配的健康人血浆中mi R-27b-3p的表达水平,同时检测其中12例胃癌患者和12例健康人外周血细胞的mi R-27b-3p表达水平。统计分析血浆mi R-27b-3p水平与胃癌患者临床病理特征的关系。运用受试者工作特征(receiver operating characteristic,ROC)曲线及曲线下面积(area under the curve,AUC)分析mi R-27b-3p对胃癌诊断的敏感度和特异度。结果:相对于健康人组,胃癌患者中血浆miR-27b-3p水平明显降低(P<0.05);而胃癌患者与健康人外周血细胞的miR-27b-3p表达水平无明显差异(P>0.05);miR-27b-3p水平下调与胃癌分化程度降低相关(P<0.05),而与胃癌TNM分期、淋巴结转移及远程转移均无关(P>0.05)。mi R-27b-3p的ROC曲线分析结果显示,AUC值为0.724;以血浆mi R-27b-3p来鉴别诊断胃癌患者的灵敏度为60.0%,特异性为76.1%。结论:血浆mi R-27b-3p水平下调可能预示着胃癌的发生。推测mi R-27b-3p可能成为胃癌早期诊断的一个生物学标志物。

Objective： To investigate the potential role of plasmatic microRNA- 27b-3p （miR-27b-3p） as a biomarker in early diagnosis of gastric cancer （GC）. Methods： The expressions of miR-27b-3p in plasma from 46 GC patients and 40 age- and gender-matched healthy controls were detected by real-time fluorescent quantitative-PCR （RFQ-PCR）. The expression of miR-27b-3p in peripheral blood cells from 12 GC patients and 12 healthy controls were also detected by RFQ-PCR. The association of plasmatic miR-27b-3p level with the clinicopathological features of GC was statistically analyzed. The sensitivity and specificity of miR-27b-3p for diagnosis of GC were evaluated by receiver operating characteristic （ROC） curve and area under the curve （AUC）. Results： The level of plasmatic miR-27b-3p in GC patients was significantly lower than that in the healthy controls （P 〈 0.05）, but the miR-27b-3p levels in peripheral blood cells had no significant difference between GC patients and the healthy controls （P 〉 0.05）. The downregulation of plasmatic miR-27b-3p level was correlated to the differentiation of GC （P 〈 0.05）, but not correlated to TNM stage, lymph node metastasis and distant matastasis （all P 〉 0.05）. The ROC curve analysis showed that AUC value was 0.724, and the sensitivity and specificity of plasmatic miR-27b-3p used for differential diagnosis of GC were 60.0% and 76.1%, respectively. Conclusion： Downregulation of plasmatic miR-27b-3p level may indicate the occurrence of GC. MiR-27b-3p may be a potential biomarker for early diagnosis of GC.