国产多孔钽复合MG63细胞培养Col-1、OC和OPN蛋白表达及意义

Expression of type I collagen,osteocalcin,and osteopontin in MG63 osteoblasts cultured with domestic porous tantalum

[目的]观察国产多孔钽材料复合MG63细胞培养后细胞增殖粘附情况,并检测成骨相关因子Col-1、OC和OPN表达情况,研究国产多孔钽材料的生物相容性,为国产多孔钽的临床应用提供理论依据。[方法]将MG63细胞与国产多孔钽材料复合培养,通过倒置相差显微镜、扫描电镜观察检测细胞粘附情况,MTT法检测细胞增殖情况,免疫细胞化学和免疫蛋白印迹法,检测Ⅰ型胶原(collagen typeⅠ,Col-1)、骨钙素(osteocalcin,OC)和骨桥蛋白(osteopontin,OPN)表达变化情况。[结果]MG63细胞与多孔钽材料复合培养后,细胞增殖情况良好,与对照组比较无差异(P>0.05);细胞在材料表面及孔隙内生长粘附并长入孔隙中,并逐渐完全覆盖材料;免疫细胞化学与免疫蛋白印迹结果提示复合培养组出现Col-1、OC表达增强(P<0.05),OPN的表达无明显差异(P>0.05)。[结论]国产多孔钽材料有利于MG63细胞的黏附、生长,具备良好的生物相容性,并可能影响Col-1、OC的表达。

[ Objective] The aim was to examine the cell proliferation and adhesion properties of MG63 ceils co- cultured with domestically produced porous tantalum material, and the expression of type I collagen, osteocalcin, and osteopontin to study the biocompatibility of the porous tantalum, and to provide theoretical basis for clinical application of the porous tantalum. [ Methods ] MG63 ceils cuhured with domestic porous tantalum material were used for this study. Inverted phase contrast mi- croscopy and scanning electron microscopy were used to observe cell proliferation and adhesion properties of the ceils. MTr assay was used to assess the proliferation of MG63 cells co - cultured with porous tantalum material, and immunocytochemistry and western blot analysis were used to assess the expression of type [ collagen, osteocalcin, and osteopontin. [ Results ] MG - 63 cells co - cultured with porous tantalum material grew well, and there was no difference in the growth compared with that of cells in the control group. Cells seeded on the surface of the porous material adhered, proliferated, and filled the pores, gradually covering the material completely. Results of immunocytochemistry and western blotting showed that the expression of type I colla- gen and osteocalcin increased in cells co - cultured with domestic porous tantalum material as compared with the control group （P 〈 0. 05）, while the expression of osteopontin was similar for the control and test cells. [ Conclusion ] Domestic porous tan- talum material promotes MG63 cell adhesion and proliferation, and provides good biocompatibility, thereby promoting the ex- pression of type I collagen and osteoealcin.