骨皮质切开术辅助大鼠正畸牙移动中OPNmRNA、BSPmRNA、OCNmRNA表达的研究

Research of OPNmRNA,BSPmRNA,OCNmRNA Expression during Corticotomy Assisted Orthodontic Tooth Movement in Rats

目的:观察骨桥蛋白(OPN),骨涎蛋白(BSP),骨钙蛋白(OCN)在大鼠骨皮质切开术辅助正畸牙移动过程中的表达情况。方法:选取健康雄性Wistar大鼠65只,60只大鼠随机分成3组:组Ⅰ:选择性骨皮质切开术;组Ⅱ:传统的牙齿移动;组Ⅲ:混合组,剩余5只作为空白对照组(不作任何处理)。用镍钛螺旋拉簧施加约60g力近中移动上颌实验侧第一磨牙,在第一磨牙颊腭侧行骨皮质打孔术式,分别在加载第7、14、21、28、42天颈椎脱臼处死取材,进行SYBR Green实时荧光定量PCR技术,对Ct值进行分析。结果:混合组中的成骨细胞相关细胞因子:OPN(骨桥蛋白),Bone sialoprotein(BSP骨涎蛋白),Osteocalcin(OCN骨钙蛋白)的表达早期增加暗示了同化合成反应的增加。结论:正畸牙齿移动初期骨皮质切开术加速了牙齿移动,改变了早期骨代谢状态,成骨活跃,支持区域性骨代谢加速学说。

Objective： To study the expression of Osteoponfin （OPN） ,bone sialoprotein （BSP） ,osteocalein （OCN） during corticotomy assisted orthodontic tooth movement in rats. Methods： A total of 65 only eight--week healthy male Wistar rats,60 were randomly divided into 3 groups： group Ⅰ： selective corticotomy; group Ⅱ ： traditional tooth movement; group Ⅲ： mixed group; the remaining five was blank control group. Tooth movement was performed on the first molar using about 60g Ni-Ti spring. Surgery corticotomy drilling operation was performed around the buecal and palatal aspects of the left maxillary first molar tooth. On days 7,14,21,28and42, after the rats were executed by cervical dislocation, SYBR Green real--time fluorescent quantitative PCR technology was used to analyze the experimental sample, to observe the changes of bone reconstruction biochemical indicators by corticoto- my during tooth movehaent during analyzing the Ct value. Results： Osteoblasts in mixed group related cytokines： OPN （osteopontin）, BSP （Bone sialoprotein）, Osteocalcin （OCN Osteocalcin） expression of early hints at the as- similation of synthetic reaction increases. Conclusion： In the early of orthodontic tooth movement corticotomy accel- erated the teeth to move, changed the early bone metabolic state such as active ossification, supporting the theory of regional aeceleratory phenomenon.