小黑杨碳酸酐酶家族基因表达特性分析

Expression analysis of carbonic anhydrase gene family in Populus simonii × P. nigra

本文克隆了小黑杨CA家族的6个基因成员,分别命名为Psn CA1、Psn CA2、Psn CA3、Psn CA4、Psn CA5和Psn CA6,其中Psn CA4和Psn CA6属于α碳酸酐酶家族,Psn CA1、Psn CA3和Psn CA5属于β碳酸酐酶家族,而Psn CA2属于Lbeat H超基因家族。系统进化分析表明,亲缘关系较近的是Psn CA2、Psn CA4和Psn CA6,而Psn CA1、Psn CA3和Psn CA5聚为一大类。通过实时荧光RT-PCR技术研究了小黑杨根、茎、叶中Psn CA基因的表达模式,结果表明:除Psn CA6外,其余5个基因在叶部的表达量显著高于根和茎。在叶部表达量最高的为Psn CA1,在茎部和根部表达最高的为Psn CA3。Psn CA在各部位的表达均受暗处理的影响,其中各基因在根部的表达变化最大,其次为茎和叶。

In this study, we isolated six members of the CA family of genes, namely PsnCA1,PsnCA2,PsnCA3, PsnCA4,PsnCA5 and PsnCA6 respectively in Populus simonii × P. nigra. Of which PsnCA4 and PsnCA6 belonged to the family of αcarbonic anhydrase,and PsnCA1,PsnCA3 and PsnCA5 belonged to the family of β carbonic anhydrase,and PsnCA2 belonged to LbeatH super gene family. The results of phylogenetic classification showed that PsnCA1, PsnCA3 and PsnCA5 were clustered into one category, while PsnCA2, PsnCA4 and PsnCA6 belonged to another category. Real-time PCR was used to investigate the PsnCA gene expression pattern in roots, stems and leaves, and the results shows：except PsnCA6, the remaining five gene expression levels in the leaves were significantly higher than the roots and stems. The highest expression in the leaves was PsnCA1 , and the highest expression in the stems and roots was PsnCA3 . PsnCA expression governed by the dark treatment in various tissues, the largest gene expression changed in roots,followed by the stems and leaves.