CXC类趋化因子12及其受体在口腔扁平苔藓中的表达

The study on the expression of CXCL12 and CXCR4 in OLP

目的:检测CXCL12及CXCR4在口腔扁平苔藓中(OLP)的表达及分布。方法:将31例OLP黏膜标本按病损类型分型、分级,免疫组化检测CXCL12及CXCR4的表达,测量CXCL12及CXCR4的累积光密度值(IOD)。结果:1OLP组织中可以检测到CXCL12及CXCR4表达,其表达向表层扩展且逐渐增强。2依临床分型分组,网纹组、糜烂组与对照组差异均具有统计学意义(P<0.05);网纹型与糜烂型相比,CXCL12的IOD降低,CXCR4的IOD升高,网纹组与糜烂组差异无统计学意义(P>0.05)。3CXCL12及CXCR4依临床分级分组,5组之间的差异没有统计学意义(P>0.05),且临床分级与IOD无明显相关性(P>0.05)。4CXCL12的IOD与CXCR4的IOD呈正相关。结论:CXCL12及CXCR4在OLP中分布及表达的差异,提示CXCL12/CXCR4轴在OLP的发生发展中可能起到一定作用。

Objective：To explore the expression of CXCL12 and CXCR4 in normal oral mucosa and OLP of different clinical classifications at the level of protein. Method：OLP specimens（experimental group） were grouped respectively according to clinical classification and the score of clinical manifestation. The expressions of CXCL12 and CXCR4 were observed in different tissues using IH. Result：1According to the clinical classifications,the expressions of CXCL12 and CXCR4 in general group and erosive group were both higher compared to the normal oral mucosa,differences were considered statistically significant（P〈0.05）.The dyeing sites of CXCL12 and CXCR4 in general group were similar to that of erosive group,but the staining intensity of CXCL12 was weaker while that of CXCR4 was stronger. The difference of the expression between general group and erosive group was not statistically significant（P〉0.05）. 2Both CXCL12 and CXCR4 had no statistically significant differences among five groups based on the clinical manifestation（P〉0.05） and the IOD of both CXCL12 and CXCR4 had no obvious correlation with the clinical manifestation（P〉0.05）.3The staining intensity of CXCL12 had positive correlation with that of CXCR4. Conclusion：The enhancement and distribution of CXCL12 and CXCR4 expression in OLP suggested CXCL12/CXCR4 may be involved in OLP development and progression.