垂盆草提取物改善重症急性胰腺炎肺损伤的实验研究

Sedum sarmentosun Bunge Extraction Ameliorated Severe Acute Pancreatitis-induced Lung Injury: an Experimental Research

目的探讨垂盆草提取物(SSBE)对重症急性胰腺炎(SAP)模型大鼠急性肺损伤(ALI)及过度炎症反应的影响。方法将42只健康成年SD大鼠随机分成3组:假手术组(C)、胰腺炎模型组(SAP)及SSBE治疗组,每组14只。逆行胰胆管穿刺注射5%牛磺胆酸钠(1 m L/kg)制备SAP肺损伤大鼠模型,SSBE组在此基础上即刻皮下注射SSBE(100 mg/kg),12 h后同等剂量再注射1次,C组及SAP组给予等量生理盐水注射。造模成功后分别于12、24 h取标本并处死大鼠,光镜下观察胰腺及肺脏病理损伤进行评分,检测各组大鼠腹水及血清淀粉酶(AMS)、肺脏组织湿干比重(W/D)、髓过氧化物酶(MPO)活性及炎性因子(IL-1、IL-6、TNF-α)含量。结果与C组比较,SAP组腹水及血清AMS明显升高(P<0.05);肺组织MPO、IL-1、IL-6、TNF-α含量及W/D比值亦明显升高(P<0.05)。与SAP组比较,SSBE组大鼠12、24 h腹水及血清AMS、肺组织MPO、IL-1、IL-6及TNF-α、W/D比值等指标明显降低(P<0.05,P<0.01)。光镜下观察SSBE组大鼠胰腺组织及肺组织病理损害减轻,其损伤程度介于C组和SAP组之间。结论 SSBE对SAP模型大鼠肺损伤有缓解作用,可能通过减轻SAP大鼠的炎症反应而改善其ALI。

Objective To explore the effect of Sedum sarmentosum Bunge Extract （SSBE） on se- vere acute pancreatitis （SAP） induced acute lung injury （ALl） model rats and their excessive inflammatory reactions. Methods Forty-two healthy adult male Sprague-Dawley （SD） rats were randomly divided into 3 groups, the sham-operated control group （C）, the SAP group （SAP）, and the SSBE treated group （SSBE）, 14 in each group. SAP induced ALl rat model was induced by retrograde injection of 5% sodium taurocholate （1 mL/kg） into the pancreatic duct. SSBE （100 m/kg） was administrated subcutaneously after the establishment of the SAP model. Equal dose of SSBE was injected again 12 h later. Equal volume of normal saline was administrated in the same way for rats in the C group and the SAP group. Rats were sacrificed after successful modeling and samples taken at 12 and 24 h. Pathological changes in the pancreas and the lung tissue were observed under light microscope. The ascites, serum amylase （AMS）, wet/dry proportion （W/D） of the lung tissue, activities of myeloperoxidase （MPO）, interleukin-1 （IL-1）, IL-6, and tumor necrosis factor-a （TNF-oL） were also measured. Results Ascites and serum AMS activities signifi- cantly increased; MPO, IL-1, IL-6, TNF-a contents, and W/D ratio also significantly increased in the SAP group, when compared with the C group （P 〈0.05）. Compared with the SAP group, those parameters were all attenuated in the SSBE group at 12 and 24 h （P 〈0.05, P 〈0.01 ）. Pathological changes in the pancreas and the lung tissue were alleviated in the SSBE group under light microscope. The injury degree ranged be- tween that of the C group and the SAP group. Conclusion SSBE could relieve the ALl in SAP model rats, which could be achieved through alleviating inflammation responses of SAP rats.