穿山龙总皂苷对白介素-1β诱导的成纤维样滑膜细胞基质细胞衍生因子-1及IκB激酶表达的影响

Effect of Total Saponins from Rhizoma Dioscoreae nipponicae on the Expression of SDF1 and IκB Kinase inr IL-1β Induced Fibroblast-like Synoviocytes

目的观察穿山龙总皂苷(Rhizoma Dioscoreae nipponicae,RDN)对体外白介素-1β(r IL-1β)诱导的成纤维样滑膜细胞(FLS)基质细胞衍生因子-1(SDF1)及IκB激酶(IKK)表达的影响。方法原代培养FLS,取第3代对数生长期的细胞将其分为正常组、模型组及给药组,模型组给予10μg/L r IL-1β诱导FLS增殖,给药组给予10μg/L r IL-1β及100μg/L穿山龙总皂苷共同孵育,正常组不予处理,孵育72 h后采用Western blot法测定各组大鼠FLS中SDF1及IKK(p-IKK)的表达水平。结果与正常组比较,模型组SDF1及p-IKK表达水平明显升高(P<0.01);与模型组比较,给药组FLS中SDF1及p-IKK表达水平明显降低(P<0.01)。结论穿山龙总皂苷可抑制SDF-1及IκB激酶的激活,可能通过对SDF-1的表达调控进而调控IKK的表达。

Objective To study the effect of total saponins from Rhizoma Dioscoreae nipponicae （RDN） on the expression of stroma cell derived factor 1 （SDF1） and IKB kinase （IKK） in rlL-1β induced fibroblast-like synoviocytes （FLS）. Methods FLS were primarily cultured and the 3rd generation log phase growth FLS were divided into the normal control group, the model group, and the medication group. 10 μ g/L rlL-1β was used to induce the proliferation of FLS in the model group. 10 μg/L rlL-1β and 100 μg/L RDN were administered to co-incubate FLS in the medication group. No treatment was given to FLS in the normal control group. Expression levels of SDF1 and IKB kinase proteins （p-IKK） were detected using Western blot. Results Expression levels of SDF1 and p-IKK increased significantly higher in the model group than in the normal control group （P 〈0.01 ）. Compared with the model group, expression levels of SDF-1 and p-IKK significantly decreased in the medication group （P 〈0.01 ）. Conclusions Total saponins from RDN could inhibit the activation of both SDF1 and p-IKK. It might further regulate the expression of IKB kinase by regulating the expression of SDFI.