22种中草药提取物体外抗菌实验研究

Study on the Antibacterial Activities of 22 Herbal Extracts in vitro

目的：测定芸香草等22种中草药提取物体外抑菌活性。方法：乙醇冷浸制备22种植物药材的乙醇提取物,观察其对金黄色葡萄球菌、大肠埃希菌、白念珠菌、铜绿假单胞菌及耐甲氧西林金黄色葡萄球菌（MRSA）的抑制作用,用琼脂打孔法对其抗菌活性进行筛选,通过倍比稀释法测定其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果：22种乙醇提取物中有6种对金黄色葡萄球菌、大肠埃希菌、白念珠菌、铜绿假单胞菌有不同程度的抑制作用,有7种对MRSA有较强的抑制作用,抑菌圈直径为15~27 mm,MIC为31.25~500 mg/L,MBC为250~2 000 mg/L,对白念珠菌、大肠埃希菌活性较弱。结论：芸香草、肉豆蔻等7种对所测金黄色葡萄球菌及MRSA具有较好的抑菌效果。

Objective： To determine the antibacterial activities of 22 herbal plant extracts in vitro. Methods： Ethanol immersion was employed to prepare 22 herbal plant extracts. Staphylococcus aureus （SA） , Escherichiat coli （ E. Coli ）, Candida albicans （ C. Albicans ）, Pseudomonas aeruginosa （ P. Aeruginosa） and methicillin-resistant staphylococcus aureus （MRSA）. Agar hole diffusion test was applied to screen the antibacterial activity. The minimum inhibitory concentration （ MIC ） and mini- mum bactericidal concentration （MBC） were determined by double dilution method. Results： Six ex- tracts showed different inhibitory effect against SA, E. coli, P. aeruginosa and C. albicans. Seven ex- tracts showed strong inhibitory effect anti-MRSA. Their inhibition zone diameters （IZD） against were 15 -27 ram, and MIC was 31.25 -500, MBC was 250 -2 000 g/mL. These plants showed weak in- hibition against E. coli and C. albicans. Conclusion： Seven Chinese herbal extracts including Cymbo- pogon distains and Myristica fragrans showed inhibitory effects on SA and MRSA.