硫辛酸对LPS诱导的帕金森病小鼠黑质多巴胺能神经元损伤的影响

Lipoic acid protects dopaminergic neurons in LPS-induced model of Parkinson's disease

目的:探讨硫辛酸(LA)对脂多糖(LPS)诱导的帕金森病(PD)模型小鼠黑质多巴胺(DA)能神经元损伤的作用及机制。方法:10月龄雌性C57BL/6小鼠,随机分为生理盐水对照组、PD模型组和LA干预组。采用鼻腔滴入LPS制作PD小鼠模型,通过爬竿实验及酪氨酸羟化酶和p-NF-κB/p65的免疫组化技术观察LA对DA能神经元的保护作用。结果:LPS经鼻可以成功诱导小鼠PD模型,给予LA可明显恢复小鼠运动,减少黑质DA能神经元的丢失、抑制小胶质细胞及其NF-κB信号通路激活。结论:LA干预可缓解LPS经鼻诱导的小鼠PD行为学和病理学的改变,其作用机制可能与抑制脑内的炎症反应有关。

AIM： To evaluate the effect of lipoic acid（ LA） on LPS-induced Parkinson disease（ PD） model of mice. METHODS： Female C57 BL /6 mice of 10-month-old were randomly divided into saline control group,PD group and LA group. The PD mouse model was induced by intranasal instillation of LPS. Assays of tyrosine hydroxylase,microglia and nuclear factor kappa B（ NF-κB） were performed by the methods of immunohistochemistry and Western blotting. RESULTS： Intranasal LPS instillation exhibited basic characteristics of PD model. However,LA administration significantly improved motor dysfunction,protected dopaminergic neurons from damage,and inhibited NF-κB activation in inflammatory microglia in the substantia nigra area of the brain. CONCLUSION： LA may exert a profound neuroprotective effect by anti-neuroinflammatory reaction to arrest the progression of PD.