正畸治疗中牙龈蛋白K与牙龈炎性反应的关系

Detection of gingipain K in the gingival crevicular fluid of orthodontic patients

目的:分析牙龈蛋白K(gingipain K,Kgp)在正畸治疗中对牙龈炎性反应的影响。方法:选择青少年牙龈健康者45例,分别取矫治器戴入前与矫治器戴入后3个月的龈沟液,应用16S r DNA PCR技术检测各样本中的牙龈卟啉单胞菌(P.g)及Kgp,采用SPSS17.0软件包对数据进行统计学分析。结果:矫治器戴入前Kgp检出率为35.71%,矫治器戴入后Kgp检出率为67.86%,两者之间差异显著(P<0.05)。结论:正畸治疗患者在矫治器戴入后Kgp检出率增加,出现牙龈炎性反应。

PURPOSE： To explore the correlation between gingipain K（Kgp） and inflammatory reaction of gum in the process of orthodontic treatment, and analyse the role of Kgp in the development of gingivitis during orthodontic treatment.METHODS： Totally 45 orthodontic healthy teenagers were randomly chosen for the study. The subgingival plaques were collected simultaneously before orthodontic treatment and 3 months after treatment. 16 S r DNA PCR technique was used to detect Porphyromonas gingivalis（P.g） and Kgp. SPSS17.0 software package was used for statistical analysis. RESULTS：The detection rate of Kgp was 35.71% before orthodontic treatment and 67.86% after treatment. Positive correlation（ P0.05） was observed between Kgp and gingival inflammation. CONCLUSIONS： Fixed orthodontic appliances cause plaque accumulation, accordingly slight gingiva inflammation and the increament of P.gingivalis in the early stage.