生物陶瓷复合骨髓基质干细胞及骨形态发生蛋白修复兔大段桡骨缺损(英文)

Bio-ceramic combined with bone marrow stromal stem cells and bone morphogenetic protein in repair of large segmental radius defects in rabbits

背景:人工合成生物陶瓷作为良好的生物支架材料与自体骨髓基质干细胞复合并联合诱导成骨物质移植的骨组织工程为大段骨缺损的患者带来了新希望。目的:观察生物陶瓷复合骨髓基质干细胞及骨形态发生蛋白修复兔大段桡骨骨缺损的疗效及可行性。方法:实验植入兔双上肢桡骨中段制造长约1.5 cm的大段骨缺损模型,将左侧骨缺损区作为实验组,植入生物陶瓷与骨髓基质干细胞+骨形态发生蛋白;右侧骨缺损区作为对照组,单纯植入生物陶瓷。建模后4,8,12,24周各时间点对各组动物行标本大体观察、X射线片观察及组织学观察,比较其骨缺损区修复愈合情况。结果与结论:建模后4,8,12,24周,与对照组相比,实验组兔桡骨骨缺损区修复较快,新骨形成量多,塑性完全,原生物陶瓷破碎吸收现象明显。建模后24周,桡骨骨缺损区与骨端完全桥接,骨质基本得到修复。结果证实,生物陶瓷复合骨髓基质干细胞及骨形态发生蛋白作为复合移植材料治疗兔桡骨大段桡骨缺损疗效较好,效果优于单纯生物陶瓷移植材料。

BACKGROUND： Synthetic bio-ceramics as good biological scaffolds can compound with autologous bone marrow stromal stem cels. Bone tissue engineering of their combination for bone graft brings a new hope for large bone defects. OBJECTIVE：To observe the therapeutic effects and feasibility of bio-ceramic combined with bone marrow stromal stem cels and bone morphogenetic protein in repair of large bone defects in rabbits. METHODS： Rabbit models of 1.5 cm-long bone defects were made by implanting the middle radial bone in the upper limbs. Left bone defect region served as the experimental group, implanting with bio-ceramic combined with bone marrow stromal stem cels and bone morphogenetic protein. Right bone defect region served as the control group, only implanting with bio-ceramic. At 4, 8, 12 and 24 weeks after model establishment, general observation was performed in both groups. Histological changes were observed with radiographs to compare the repair and healing in the bone defect region. RESULTS AND CONCLUSION：At 4, 8, 12 and 24 weeks after model establishment, compared with the control group, the repair of bone defects was faster, new bone formation was more, with complete plasticity, broken absorption was more apparent in the experimental group. At 24 weeks, bone defect region was completely bridged with broken ends of fractured bone. Bone was basicaly repaired. Results confirmed that bio-ceramic combined with bone marrow stromal stem cels and bone morphogenetic protein as graft materials in treatment of large bone defects of rabbits had a good therapeutic effect, and the effects were better than bio-ceramic alone.