钛酸钡压电陶瓷涂层的生物相容性评价

Biocompatibility of barium titanate piezoelectric ceramic coatings

背景:课题组利用等离子喷涂技术在羟基磷灰石表面喷涂钛酸钡制备了压电陶瓷涂层,但其生物相容性尚不清楚。目的:检测钛酸钡压电陶瓷涂层的生物相容性。方法:1溶血实验:在兔抗凝血中分别加入受试样品浸提液、生理盐水与蒸馏水,检测溶血率。2短期全身毒性实验:对Wistar大鼠分别灌胃给予受试样品浸提液与生理盐水,观察动物体质量变化。3热源实验:自新西兰兔耳缘静脉分别注射受试样品浸提液与生理盐水,观察动物体温变化。4致敏实验:将豚鼠随机分为2组,实验组以材料浸提液与完全弗氏佐剂为供试液,对照组以生理盐水溶液与完全弗氏佐剂为供试液。按照GBT16886.10-2005医疗器械生物学评价第10部分:刺激与迟发型超敏反应试验规定,用最大剂量法进行致敏实验。结果与结论:受试材料钛酸钡压电陶瓷涂层无溶血作用、无毒性、不致热、无致敏作用,结果表明钛酸钡压电陶瓷涂层具有良好的生物相容性。

BACKGROUND：The research group have used plasma spraying technique to prepare a piezoelectric ceramic coating on the surface of hydroxyapatite, but the biocompatibility of this new material is not clear. OBJECTIVE：To detect the biocompatibility of barium titanate piezoelectric ceramic coatings prepared by plasma spraying technique. METHODS： （1） Hemolysis test： The leaching solution of test samples, saline and distiled water were added into the rabbit anti-clotting, to detect the rate of hemolysis. （2） Short-term systemic toxicity test： Wistar rats were intragastricaly given the leaching solution of test samples and normal saline, to observe the animal body weight changes. （3） Pyrogen test： The leaching solution of test samples and saline were injected into the ear vein of New Zealand rabbits, to observe the changes of animal body temperature. （4） Sensitization test： The guinea pigs were randomly divided into two groups, and respectively given the leaching solution of test samples and complete Freund’s adjuvant （experimental group） as wel as the saline solution and complete Freund’s adjuvant （control＆amp;nbsp;group）. In accordance with the stimulus and delayed-type hypersensitivity test requirements in GBT16886.10-2005 Part 10, the maximum dose was used for sensitization test. RESULTS AND CONCLUSION：The barium titanate piezoelectric ceramic coatings have no hemolytic effect, non-toxicity, no sensitization, and cannot cause hot, indicating that the biological piezoelectric ceramic coating has good biocompatibility.