天然药物表没食子儿茶素浸食子酸酯与牙本质龋的再矿化

Effect of epigallocatechin gallate on remineralization of dentine caries

背景:研究发现表没食子儿茶素浸食子酸酯具有抑制免疫炎症反应、抗菌、抗氧化、抗突变和抗癌等多种功效。目的:通过体外实验探讨表没食子儿茶素浸食子酸酯对牙本质龋再矿化方面的影响。方法:将30颗正畸拔除的人离体牙按照随机数字表均分为实验组、对照组及空白对照组,在乳酸脱矿系统制成牙本质龋后,分别置于2 g/L表没食子儿茶素浸食子酸酯溶液、饱和Ca(OH)2溶液、人工唾液中12 d进行再矿化实验,测定3种溶液中牙本质块表面显微硬度,扫描电镜观察牙本质块表面再矿化结果。结果与结论:按照再矿化后牙本质表面显微硬度从高到低的顺序依次排列为:对照组、实验组、空白对照组,组间两两比较差异均有显著性意义(P<0.05),结果表明表没食子儿茶素浸食子酸酯与Ca(OH)2在牙本质再矿化方面的作用好于人工唾液,两者均能促进牙本质龋的再矿化,且表没食子儿茶素浸食子酸酯促进牙本质龋再矿化效果低于Ca(OH)2。扫描电镜显示,对照组牙本质表面附着有大量沉积物,未见牙本质小管开口;实验组也可见沉积物附着牙本质表面,但较平整;空白对照组牙本质表面沉积物较少,可见有未覆盖沉积物的牙本质小管口。扫描电镜结果定性证明了表没食子儿茶素浸食子酸酯能促进脱矿牙本质的再矿化。

BACKGROUND：Epigalocatechin galate can inhibit immune-inflammatory responses, and have anti-bacterial, anti-oxidation, anti-mutagenic and anti-cancer effects. OBJECTIVE： To evaluate the effects of the natural medicine epigalocatechin galate on the remineralization of dentin caries through in vitro experiments. METHODS： A total of 30 extracted teeth for orthodontic reason were randomly divided into three groups： experimental group, control group and blank control group. After dentin caries were induced by lactic acid gel, the extracted teeth were immersed into 2 g/L epigalocatechin galate solution, saturated Ca（OH）2 and artificial saliva, respectively, for 12 days. After treatments, the morphological structure of dentin surfaces were observed by scanning electron microscope, and the micro hardness of dentin surfaces was measured in three groups. RESULTS AND CONCLUSION：The microhardness of remineralized dentin surfaces was ranged as folows： control group 〉 experimental group 〉 blank control group, and there were significant differences between groups （P 〈 0.05）. This indicated that epigalocatechin galate and Ca（OH）2 were better than the artificial saliva in the dentine remineralization, and moreover, epigalocatechin galate was inferior to Ca（OH）2 in the dentine remineralization. Under the scanning electron microscope, there were a lot of sediments on the dentin surfaces of the control group, but the dentinal tubule openings were not seen; the sediments of the experimental group were also seen on the dentine surfaces, and the surfaces were even; the least sediments were found in the blank control group, and there were some dentinal tubule openings that were not covered by sediments. The results of scanning electron microscope qualitatively demonstrate that the epigalocatechin galate can promote the dentine remineralization.