含纳米SiO_2或纳米TiO_2微弧氧化纯镁生物涂层可促进成骨细胞增殖及活性

Pure magnesium micro-arc oxidation coating containing nano-SiO_2 or nano-TiO_2 promotes proliferation and viability of osteoblasts

背景:微弧氧化技术可增强镁及其合金的耐腐蚀性,提高其表面生物性能。目的:为了调控医用纯镁的生物活性,在镀液中添加纳米Si O2或纳米Ti O2对纯镁微弧氧化涂层改良,研究其对成骨细胞增殖及分化的影响。方法:将圆形镁片分为3组,其中两组分别置入含7.5 g/L纳米Si O2或4.8 g/L纳米Ti O2的硅酸盐电解液中进行表面微弧氧化处理,以未作任何处理的纯镁作为对照。将第3代成骨细胞分别接种于3组试件表面,观察成骨细胞的早期形态、增殖与碱性磷酸酶活性。结果与结论:成骨细胞在纳米SiO 2组、纳米Ti O2组试件表面生长状态良好,轮廓清晰,呈长梭形,多角形;在对照组表面生长状态较差。CCK-8检测显示,3组细胞吸光度值与碱性磷酸酶活性随时间推移呈上升趋势,纳米Si O2组、纳米Ti O2组试件接种1,3,5 d的细胞增殖活性高于对照组;纳米Si O2组、纳米Ti O2组接种3,5 d的细胞碱性磷酸酶活性高于对照组。结果表明纳米Si O2或纳米TiO 2微弧氧化生物涂层可促进成骨细胞增殖及成骨活性,具有良好的生物相容性。

BACKGROUND：The micro-arc oxidation technology can enhance the corrosion resistance of magnesium and its aloys to improve the biological properties of the surface. OBJECTIVE： In order to regulate the biological activity of medical pure magnesium, to modify the micro-arc oxidation coating by adding nano-SiO2 and nano-TiO2 into the plating solution, and to study the biological coating effects on osteoblast proliferation and differentiation. METHODS： Round magnesium sheets were divided into three groups, respectively treated with silicate electrolyte containing 7.5 g/L nano-SiO2 （nano-SiO2 group）, 4.8 g/L nano-TiO2 （nano-TiO2 group）, and nothing （control group）. Passage 3 osteoblasts were seeded onto the specimen surface in the three groups to observe the early morphology, proliferation and alkaline phosphatase activity of osteoblasts. RESULTS AND CONCLUSION：Osteoblasts grew wel in the nano-SiO2 group and nano-TiO2 group, presenting with clear outline, and the cels were long spindle- and polygon-shaped. But in the control group, the cels grew poor in the specimen surface. Cel counting kit-8 test showed that the absorbance values and alkaline phosphatase activities were increased with time in the three groups. Compared with the control group, the＆amp;nbsp;proliferative activity was higher in the nano-SiO2 group and nano-TiO2 group at days 1, 3, 5 after seeding, while the activity of alkaline phosphatase was also higher in the nano-SiO2 group and nano-TiO2 group at days 3, 5 after seeding. These findings indicate the micro-arc oxidation coatings containing nano-SiO2and nano-TiO2 can promote osteoblast proliferation and osteogenetic activity, which have good biocompatibility.