不同性别肾缺血再灌注损伤模型小鼠MicroRNA的表达谱

Micro RNA expression profiling in male and female model mice after renal ischemia-reperfusion injury

背景:有研究表明肾缺血再灌注损伤存在性别差异,但其具体机制有待进一步研究。目的:观察雌雄性小鼠缺血前后肾脏组织中Micro RNA表达及其差异,以期进一步研究Micro RNA在肾缺血再灌注损伤性别差异中的作用及机制。方法:将雄性和雌性小鼠分别肾缺血45 min再灌注损伤24 h,同时设置雄性和雌性假手术组作对照。采用Micro RNA基因芯片技术检测雌雄小鼠肾缺血45 min再灌注损伤24 h及假手术后肾组织Micro RNA表达的差异,样品间表达差异的阈值为2倍。结果与结论:在雌性肾缺血再灌注与雄性肾缺血再灌注组对比发现有表达上调的Micro RNA有5个;雌性肾缺血再灌注组与雌性假手术组对比发现有差异表达的Micro RNA有29个,其中上调的有Micro RNA有25个,下调的Micro RNA有4个;雄性肾缺血再灌注组与雄性假手术组对比发现有差异表达的Micro RNA有38个,其中上调的Micro RNA有9个,下调的Micro RNA有29个;雌性假手术组与雄性假手术组对比发现有差异表达的Micro RNA有102个,其中上调Micro RNA的有22个,下调的Micro RNA有80个。结果说明,不同性别小鼠肾缺血再灌注前后肾组织中微小核糖核酸表达存在差异,这些Micro RNA的差异表达可能导致不同性别小鼠肾脏对缺血再灌注损伤的敏感性及耐受性存在差异。

BACKGROUND：Renal ischemia-reperfusion injury has been shown to exhibit gender difference, but its precise mechanisms deserve further investigations. OBJECTIVE：To investigate the differential expression of microRNAs in the kidney between female and male mice in order to study the effects and mechanisms of microRNA in pathogenesis of ischemia-reperfusion injury between different genders. METHODS：Male and female mice received kidney ischemia for 45 minutes and reperfusion injury for 24 hours. Simultaneously, male and female sham surgery groups served as controls. The microRNA gene chip technology was used to detect the differences of microRNA expression in the kidney of male and female mice at 45 minutes after ischemia and 24 hours of reperfusion as wel as after sham surgery. The threshold of difference in expression among samples was double. RESULTS AND CONCLUSION：Five microRNAs were up-regulated between female and male ischemia-reperfusion injury groups. Twenty-nine microRNAs differential y expressed in the female ischemia-reperfusion group and female sham surgery group, including 25 up-regulated microRNAs and 4 down-regulated microRNAs. Thirty-eight microRNAs differential y expressed in male ischemia-reperfusion injury＆amp;nbsp;group and male sham surgery group, including 9 up-regulated microRNAs and 29 down-regulated microRNAs. 102 microRNAs differential y expressed in the female sham surgery group and male sham surgery group, including 22 up-regulated microRNAs and 80 down-regulated microRNAs. Results suggested that there was differential expression in microRNAs in the kidney before and after renal ischemia-reperfusion in male and female mice. These differential y expressed microRNAs may be lead to different sensitivity and tolerance to the ischemia-reperfusion injury in the kidney of male and female mice.