新风胶囊配伍前后毒性成分雷公藤酯甲的量变研究

Quantitative research on toxic component-wilforlide A in Xinfeng capsules before and after compatibility

目的 研究配伍前后新风胶囊中雷公藤酯甲含量的变化.方法 采用薄层扫描法（TLCS）法测定新风胶囊中雷公藤酯甲含量和雷公藤单煎液中雷公藤酯甲含量.展开剂：三氯甲烷-甲醇（85∶1）,扫描波长λS=230 nm.结果 雷公藤酯甲在1.700～5.100 μg范围内有良好的线性关系,回归方程为Y=1 118.766 ＋3 253.751X（r =0.9993）.新风胶囊中雷公藤酯甲平均回收率为102.84％,RSD=3.64％;雷公藤单煎液中雷公藤酯甲回收率为98.14％,相对标准偏差（RSD）为2.21％,配伍前后含量的变化（配伍前0.179 mg/g,配伍后0.136 mg/g）.结论 所建立的TLCS法简便、专属性强,可准确地进行雷公藤酯甲的定量检测,对比研究发现配伍后新风胶囊中雷公藤酯甲含量减少,可能与减毒有关.

Objective To study the content changes of wilforlide A in Xinfeng capsules before and after compatibility.Methods The content of wilforlide A was determined in Xinfeng capsules ester content and in Tripterygium wilfordii Hook.f.single solution by TLCS method.Developing solvents：chloroform and methanol （85：1）,scanning wavelength：230 nm.Results Wilforlide A showed a good linear relationship in 1.700-5.100p g,the regression equation is Y =1 118.766 ＋ 3 253.751 X （r =0.999）.The average recovery of the wilforlide A in Xinfeng capsules was 102.84%,and RSD was 3.64%.The average recovery of the wilforlide A in the Tripterygium wilfordii Hook.f.single decoction was 98.14%,and RSD was 2.21%,compatibility levels before and after the change （compatibility with 0.179mg/g,0.136mg/g after compatibility）.Conclusion The established TLCS method was simple,specific and accurately quantitative.The comparative study found that the compatibility of Xinfeng capsules Tripterygium reduced the content of wilforlide A,which may be related to decreasing toxicity.